Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N6-monomethylation and N6,N6-dimethylation of ribosomal RNA

Leonard Katz; David Brown; Kathleen Boris; James Tuan

doi:10.1016/0378-1119(87)90291-5

Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N⁶-monomethylation and N⁶,N⁶-dimethylation of ribosomal RNA

Leonard Katz, David Brown, Kathleen Boris, James Tuan

Host-Pathogen Interface Biology

Research output: Contribution to journal › Article › peer-review

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Abstract

The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N⁶-mono- and N⁶,N⁶ dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N⁶-mono- and N⁶,N⁶-dimethylated adenine residues.

Original language	English (US)
Pages (from-to)	319-325
Number of pages	7
Journal	Gene
Volume	55
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(87)90291-5
State	Published - 1987

Keywords

Recombinant DNA
adenine methylation
erythromycin resistance
heterologous gene expression
in vitro assay
phage γ vector

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Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N⁶-monomethylation and N⁶,N⁶-dimethylation of ribosomal RNA. / Katz, Leonard; Brown, David; Boris, Kathleen et al.
In: Gene, Vol. 55, No. 2-3, 1987, p. 319-325.

Research output: Contribution to journal › Article › peer-review

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title = "Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N6-monomethylation and N6,N6-dimethylation of ribosomal RNA",

abstract = "The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6 dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.",

keywords = "Recombinant DNA, adenine methylation, erythromycin resistance, heterologous gene expression, in vitro assay, phage γ vector",

author = "Leonard Katz and David Brown and Kathleen Boris and James Tuan",

year = "1987",

doi = "10.1016/0378-1119(87)90291-5",

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T1 - Expression of the macrolide-lincosamide-streptogramin-B-resistance methylase gene, ermE, from Streptomyces erythraeus in Escherichia coli results in N6-monomethylation and N6,N6-dimethylation of ribosomal RNA

AU - Katz, Leonard

AU - Brown, David

AU - Boris, Kathleen

AU - Tuan, James

PY - 1987

Y1 - 1987

N2 - The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6 dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.

AB - The ermE gene was cloned from Streptomyces erythraeus into Escherichia coli on a series of plasmids. When transcribed from the lac promoter, ermE conferred high-level resistance to erythromycin and other macrolide-lincosamide-streptogramin-B (MLS) antibiotics. A methylase activity capable of N6-mono- and N6,N6 dimethylation of adenine residues in E. coli rRNA was detected in extracts of MLS-resistant cells. In addition, rRNA extracted from MLS-resistant E. coli contained N6-mono- and N6,N6-dimethylated adenine residues.

KW - Recombinant DNA

KW - adenine methylation

KW - erythromycin resistance

KW - heterologous gene expression

KW - in vitro assay

KW - phage γ vector

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