Facile photopatterning of polyfluorene for patterned neuronal networks

Nam Seob Baek, Yong Hee Kim, Young Hwan Han, Bong Joon Lee, Tae Dong Kim, Sin Tae Kim, Young Seok Choi, Gook Hwa Kim, Myung Ae Chung, Sang Don Jung

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In this paper, we demonstrated a facile photopatterning method that uses photocrosslinkable polyfluorene to fabricate micro-sized photopatterns on transparent indium tin oxide substrate for neuronal patterning. The modified poly(ethyleneimine) (m-PEI) with trimethoxysilane moiety was chemically attached to the hydroxyl group-terminated ITO surface and then the photopatternable polyfluorene derivative was spin coated as a cell-repellent layer onto the m-PEI-coated surface. The well-defined micropatterns were easily created over an entire surface by photocrosslinking of bromoalkyl-substituted polyfluorene (Br-PF) via the radical coupling reaction of a C-Br bond under UV irradiation without an initiator. UV-Vis absorbance, photoluminescence, ATR-FTIR and X-ray photoelectron spectroscopy were used to confirm the photocrosslinking process and the surface composition before and after the photocrosslinking of polyfluorene. The pairing of adhesive m-PEI and repulsive Br-PF effectively guided the neurite outgrowth and controlled neurite extension from individual neurons to the pre-patterned direction with excellent pattern fidelity. Guided neuronal cells were maintained for at least 25 days in vitro without any detachment of neuronal cells during cell culture. A photopatternable polyfluorene derivative in combination with cell-adhesive m-PEI is proved to be an effective way to modify the electrode surface to achieve single cell level neuronal networks.

Original languageEnglish (US)
Pages (from-to)10025-10031
Number of pages7
JournalSoft Matter
Volume7
Issue number21
DOIs
StatePublished - Nov 7 2011

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