F‐actin Distribution of Dictyostelium Myosin I Double Mutants

MICHELLE D. PETERSON, MARGARET A. TITUS

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Abstract

The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA/myoB, myoB/myoC, and myoA/myoC) were examined with respect to their cytoskeletal organization. F‐actin localization by rhodamine‐phalloidin staining of cells indicates that the myoA/myoB, myoB/myoC, and myoA/myoC cells appear to redistribute their F‐actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.

Original languageEnglish (US)
Pages (from-to)652-657
Number of pages6
JournalJournal of Eukaryotic Microbiology
Volume41
Issue number6
DOIs
StatePublished - Nov 1994

Keywords

  • Cytoskeleton

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