TY - JOUR
T1 - F‐actin Distribution of Dictyostelium Myosin I Double Mutants
AU - PETERSON, MICHELLE D.
AU - TITUS, MARGARET A.
PY - 1994/11
Y1 - 1994/11
N2 - The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA‐/myoB‐, myoB‐/myoC‐, and myoA‐/myoC‐) were examined with respect to their cytoskeletal organization. F‐actin localization by rhodamine‐phalloidin staining of cells indicates that the myoA‐/myoB‐, myoB‐/myoC‐, and myoA‐/myoC‐ cells appear to redistribute their F‐actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.
AB - The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA‐/myoB‐, myoB‐/myoC‐, and myoA‐/myoC‐) were examined with respect to their cytoskeletal organization. F‐actin localization by rhodamine‐phalloidin staining of cells indicates that the myoA‐/myoB‐, myoB‐/myoC‐, and myoA‐/myoC‐ cells appear to redistribute their F‐actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.
KW - Cytoskeleton
UR - http://www.scopus.com/inward/record.url?scp=0028534963&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028534963&partnerID=8YFLogxK
U2 - 10.1111/j.1550-7408.1994.tb01529.x
DO - 10.1111/j.1550-7408.1994.tb01529.x
M3 - Article
C2 - 7866390
AN - SCOPUS:0028534963
SN - 1066-5234
VL - 41
SP - 652
EP - 657
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
IS - 6
ER -