Factors influencing dengue virus isolation by C6/36 cell culture and mosquito inoculation of nested PCR-positive clinical samples

Richard G. Jarman, Ananda Nisalak, Kathryn B. Anderson, Chonticha Klungthong, Butsaya Thaisomboonsuk, Winai Kaneechit, Siripen Kalayanarooj, Robert V. Gibbons

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Dengue viral isolation is necessary for definitive diagnosis, pathogenesis and evolutionary research, vaccine candidates, and diagnostic materials. Using standardized techniques, we analyzed isolation rates of 1,544 randomly selected polymerase chain reaction (PCR)-positive samples, representing all four dengue serotypes, from patients with serologically confirmed dengue infections and evaluated whether clinical and laboratory results could be predictive of isolation using standard and mosquito isolation techniques. Viruses were isolated from 62.5% of the samples by direct application to C6/36 cells and increased to 79.4% when amplifying C6/36 negative samples by intrathorasic inoculation in Toxyrhynchites splendens mosquitoes. High viremia, measured by reverse transcriptase (RT)-PCR, was a strong predictor for viral isolation by either method. Isolation was most successful in samples collected early in the disease, had low antibody levels, temperatures greater than 38°C, and had a final clinical diagnosis of dengue fever. Dengue serotypes also played a role in the success of viral isolation.

Original languageEnglish (US)
Pages (from-to)218-223
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume84
Issue number2
DOIs
StatePublished - Feb 2011

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