Factor(s) released by glucose-deprived astrocytes enhance glucose transporter expression and activity in rat brain endothelial cells

Anthony Régina; Stephanie Morchoisne; Nancy D. Borson; Anthony L. McCall; Lester R Drewes; Françoise Roux

doi:10.1016/S0167-4889(01)00133-1

Factor(s) released by glucose-deprived astrocytes enhance glucose transporter expression and activity in rat brain endothelial cells

Anthony Régina, Stephanie Morchoisne, Nancy D. Borson, Anthony L. McCall, Lester R Drewes, Françoise Roux

Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

Glucose transporter (GLUT) expression and regulation were studied in rat brain endothelial cells in primary culture (RBEC) and in immortalised RBE4 cells. Immunoblotting analysis showed a low expression of the endothelium-specific GLUT1 in RBEC and RBE4 cells compared to isolated brain capillaries. RBEC and RBE4 cells also expressed the GLUT3 isoform, whereas it was not present in isolated brain capillaries. No change in GLUT expression was observed in endothelial cells treated with astrocyte-conditioned medium. However, treatment with conditioned medium obtained from glucose-deprived astrocytes increased endothelial GLUT1 expression and glucose uptake. These results suggest that astrocytes submitted to hypoglycaemic conditions may release factor(s) that increase glucose uptake through the blood-brain barrier.

Original language	English (US)
Pages (from-to)	233-242
Number of pages	10
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1540
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4889(01)00133-1
State	Published - Sep 26 2001

Bibliographical note

Funding Information:
This work was supported by a grant from the Fondation pour la Recherche Médicale (FRM).

Keywords

Astrocyte
Blood-brain barrier
Brain endothelial cell
Glucose transporter
Hypoglycemia

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Factor(s) released by glucose-deprived astrocytes enhance glucose transporter expression and activity in rat brain endothelial cells. / Régina, Anthony; Morchoisne, Stephanie; Borson, Nancy D. et al.
In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1540, No. 3, 26.09.2001, p. 233-242.

Research output: Contribution to journal › Article › peer-review

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abstract = "Glucose transporter (GLUT) expression and regulation were studied in rat brain endothelial cells in primary culture (RBEC) and in immortalised RBE4 cells. Immunoblotting analysis showed a low expression of the endothelium-specific GLUT1 in RBEC and RBE4 cells compared to isolated brain capillaries. RBEC and RBE4 cells also expressed the GLUT3 isoform, whereas it was not present in isolated brain capillaries. No change in GLUT expression was observed in endothelial cells treated with astrocyte-conditioned medium. However, treatment with conditioned medium obtained from glucose-deprived astrocytes increased endothelial GLUT1 expression and glucose uptake. These results suggest that astrocytes submitted to hypoglycaemic conditions may release factor(s) that increase glucose uptake through the blood-brain barrier.",

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AU - Borson, Nancy D.

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AU - Drewes, Lester R

AU - Roux, Françoise

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AB - Glucose transporter (GLUT) expression and regulation were studied in rat brain endothelial cells in primary culture (RBEC) and in immortalised RBE4 cells. Immunoblotting analysis showed a low expression of the endothelium-specific GLUT1 in RBEC and RBE4 cells compared to isolated brain capillaries. RBEC and RBE4 cells also expressed the GLUT3 isoform, whereas it was not present in isolated brain capillaries. No change in GLUT expression was observed in endothelial cells treated with astrocyte-conditioned medium. However, treatment with conditioned medium obtained from glucose-deprived astrocytes increased endothelial GLUT1 expression and glucose uptake. These results suggest that astrocytes submitted to hypoglycaemic conditions may release factor(s) that increase glucose uptake through the blood-brain barrier.

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Factor(s) released by glucose-deprived astrocytes enhance glucose transporter expression and activity in rat brain endothelial cells

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