Our previous studies revealed that, in a murine model of asthma, mice that received Fas-deficient T cells developed a prolonged phase of airway inflammation, mucus production, and airway hyperreactivity that failed to resolve even 6 weeks after the last challenge. To investigate how Fas-Fas ligand (FasL) interaction occurs between T cells and other cells in vivo, Gld mice with abnormalities of the FasL signaling pathway were used. The reconstituted mice were made by transferring T cells from B6 or Gld mice to Rag-/- or FasL-deficient Rag-/- mice. We found that Rag-/- mice that received B6 T cells resolved the airway inflammation, whereas FasL-deficient Rag-/- mice that received Gld T cells developed a prolonged airway inflammation at Day 28, with decreased IFN-γ production. Both FasL-deficient Rag-/- mice that received B6 T cells and Rag -/- mice that received Gld T cells also had completely resolved their airway inflammation by Day 28 after challenge. Interestingly, FasL-deficient Rag-/- mice that received Gld T cells eventually resolved airway inflammation at Day 42, with a similar level of IFN-γ production to that of control group. These results demonstrate that FasL expression on either T cells only or non-T cells only was sufficient for the eventual resolution of airway inflammation, and the prolonged airway inflammation in FasL-deficient Rag-/- mice that received Gld T cells was correlated with decreased IFN-γ production by Gld T cells.
|Original language||English (US)|
|Number of pages||7|
|Journal||American journal of respiratory cell and molecular biology|
|State||Published - Sep 1 2010|
- T helper cell type 1/T helper cell type 2 cells