Fast, Efficient, and Gentle Transfection of Human Adherent Cells in Suspension

Pranav Agrawal, Nilesh P. Ingle, William S. Boyle, Emily Ward, Jakub Tolar, Kevin D. Dorfman, Theresa M. Reineke

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We demonstrate a highly efficient method for gene delivery into clinically relevant human cell types, such as induced pluripotent stem cells (iPSCs) and fibroblasts, reducing the protocol time by one full day. To preserve cell physiology during gene transfer, we designed a microfluidic strategy, which facilitates significant gene delivery in a short transfection time (<1 min) for several human cell types. This fast, optimized and generally applicable cell transfection method can be used for rapid screening of different delivery systems and has significant potential for high-throughput cell therapy applications.

Original languageEnglish (US)
Pages (from-to)8870-8874
Number of pages5
JournalACS Applied Materials and Interfaces
Volume8
Issue number14
DOIs
StatePublished - Apr 27 2016

Bibliographical note

Funding Information:
This work was supported by the David and Lucile Packard Foundation and University of Minnesota. J.T. was supported in part by R01 AR063070, R01 AR059947-01A1, DOD W81XWH-12-1-0609 and P01 CA065493. Portions of this work were performed in the University of Minnesota Nanofabrication Center, which receives a partial support from the National Science Foundation (NSF) through National Nanotechnology Infrastructure Network (NNIN).

Keywords

  • fibroblasts
  • flow cytometry
  • microfluidics
  • pluripotent stem cells
  • suspension transfection

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