Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV

M. C. Jenkins; J. Higgins; Juan E Abrahante - Llorens; K. E. Kniel; C. O'Brien; J. Trout; C. A. Lancto; M. S. Abrahamsen; R. Fayer

doi:10.1016/j.ijpara.2007.11.005

Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV

M. C. Jenkins, J. Higgins, Juan E Abrahante - Llorens, K. E. Kniel, C. O'Brien, J. Trout, C. A. Lancto, M. S. Abrahamsen, R. Fayer

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Abstract

Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 10⁶ C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum.

Original language	English (US)
Pages (from-to)	1051-1055
Number of pages	5
Journal	International Journal for Parasitology
Volume	38
Issue number	8-9
DOIs	https://doi.org/10.1016/j.ijpara.2007.11.005
State	Published - Jul 2008

Keywords

Cryptosporidium parvum
Fecundity
Oocysts
Viral symbiont
Virus

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title = "Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV",

abstract = "Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 106 C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum.",

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AU - Higgins, J.

AU - Abrahante - Llorens, Juan E

AU - Kniel, K. E.

AU - O'Brien, C.

AU - Trout, J.

AU - Lancto, C. A.

AU - Abrahamsen, M. S.

AU - Fayer, R.

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N2 - Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 106 C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum.

AB - Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 106 C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum.

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Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV

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