Feeding pasteurized waste milk to preweaned dairy calves changes fecal and upper respiratory tract microbiota

Georgina Maynou, Hugh Chester-Jones, Alex Bach, Marta Terré

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study bacterial communities from both, the gastrointestinal and respiratory tract of pre-weaned dairy calves fed two different milk-feeding programs were characterized using 16S rRNA gene sequencing. Twenty female Holstein calves (38.8 ± 1.40 kg of BW) were fed pasteurized waste milk (pWM) containing residues of various antimicrobials. Twenty additional calves (38.1 ± 1.19 kg of BW) were fed milk replacer (MR) with similar nutrient composition (27.5% crude protein, 32.1% fat) compared to waste milk (28.6% crude protein, 30.0% fat) from day 1 to weaning at day 49 of study. Fecal samples and nasal swabs were collected on day 42 only from calves that were not treated with therapeutic antibiotics throughout the study, which were 8 MR and 10 pWM calves. To assess the impact of the two feeding regimes on the fecal and nasal microbiota, α and β-diversity measures were calculated, and the relative abundance of operational taxonomic units (OTUs) at different taxonomic levels was determined for each sample. In general, Chao1, PD Whole Tree, and Shannon diversity indices were similar for the fecal and nasal bacterial communities of calves regardless of the feeding regime. However, principal coordinate analysis based on unweighted Unifrac distances indicated differences in the structure of bacterial communities of calves fed milk replacer compared with those from calves fed pasteurized waste milk. The relative abundance of the Streptococcaceae family and the genus Histophilus was greater (P < 0.05) in the nasal microbiota of calves fed milk replacer than in those fed pasteurized waste milk. However, the genus Prevotella tended (P = 0.06) to be more relatively abundant in the respiratory tract of calves fed pasteurized waste milk than in those fed milk replacer. Differences in relative abundances of bacterial taxa in gut microbiota were only observed at the phylum level, suggesting that antimicrobial residues present in waste milk have a non-specific influence at a lower taxonomical level.

Original languageEnglish (US)
Article number159
JournalFrontiers in Veterinary Science
Volume6
Issue numberJUN
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
This research was partially supported by the University of Minnesota Southern Research and Outreach Center (SROC) and Milk Products, Chilton, WI. Data from this study have been partially presented in an abstract form at the ADSA-ASAS Joint Annual Meeting in Salt Lake City, Utah, July 19-23, 2016. The authors would like to thank the excellent technical assistance of David Ziegler and My Yang from the University of Minnesota for their help with calf management and sampling processing. Furthermore, the authors specially thank Carles Borrego from the Catalan Institute for Water Research (ICRA) for his assistance with the QIIME software, the staff at the SROC Dairy Calf and Heifer Facility for their assistance in data collection, and the members of the Plant Pathology Department at SROC for facilitating the use of their laboratory during sampling procedures. We also thank the CERCA program from Generalitat de Catalunya (Barcelona, Spain) for partially supporting this research.

Publisher Copyright:
Copyright © 2019 Maynou, Chester-Jones, Bach and Terré. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

  • 16S rRNA gene sequencing
  • Calves
  • Fecal microbiota
  • Upper respiratory tract microbiota
  • Waste milk

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