TY - GEN
T1 - Fibroblast behavior on electrospun PMMAEA and PMMAEA-collagen nanofibers
AU - Hu, Wen
AU - Holy, Jon
AU - Yu, Xun
PY - 2013
Y1 - 2013
N2 - The influence of different physical forms of substrates on fibroblast behavior was examined by comparing the following experimental and control groups: 1) glass coverslips, 2) poly(methyl methacrylate-co-ethyl acrylate) (PMMAEA) cast films, 3) electrospun PMMAEA nanofibers, 4) electrospun PMMAEA/collagen nanofibers, and 5) electrospun collagen. Cell adhesion, spreading and proliferation were compared on the different substrates. It was observed that fibroblasts on electrospun PMMAEA, PMMAEA-collagen, and collagen substrates spread more slowly after plating, and did not spread out to the extent observed for glass or PMMAEA films. Cells on electrospun fibers exhibited more filopodial-like structures and fewer stress fibers than the glass and PMMAEA film surfaces. Cell viability studies showed that although cells remained viable on all substrates, proliferation was faster on glass and PMMAEA films than on electrospun substrates. Overall, fibroblast behavior appeared to more closely resemble in vivo behavior on the electrospun nanofibers than on films or glass substrates.
AB - The influence of different physical forms of substrates on fibroblast behavior was examined by comparing the following experimental and control groups: 1) glass coverslips, 2) poly(methyl methacrylate-co-ethyl acrylate) (PMMAEA) cast films, 3) electrospun PMMAEA nanofibers, 4) electrospun PMMAEA/collagen nanofibers, and 5) electrospun collagen. Cell adhesion, spreading and proliferation were compared on the different substrates. It was observed that fibroblasts on electrospun PMMAEA, PMMAEA-collagen, and collagen substrates spread more slowly after plating, and did not spread out to the extent observed for glass or PMMAEA films. Cells on electrospun fibers exhibited more filopodial-like structures and fewer stress fibers than the glass and PMMAEA film surfaces. Cell viability studies showed that although cells remained viable on all substrates, proliferation was faster on glass and PMMAEA films than on electrospun substrates. Overall, fibroblast behavior appeared to more closely resemble in vivo behavior on the electrospun nanofibers than on films or glass substrates.
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U2 - 10.1115/IMECE2013-62980
DO - 10.1115/IMECE2013-62980
M3 - Conference contribution
AN - SCOPUS:84903467074
SN - 9780791856222
T3 - ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
BT - Biomedical and Biotechnology Engineering
PB - American Society of Mechanical Engineers (ASME)
T2 - ASME 2013 International Mechanical Engineering Congress and Exposition, IMECE 2013
Y2 - 15 November 2013 through 21 November 2013
ER -