Flexible and disposable immunosensors based on layer-by-layer self-assembled carbon nanotubes and biomolecules

Miao Lu; Dongjin Lee; Wei Xue; Tianhong Cui

doi:10.1016/j.sna.2008.12.021

Flexible and disposable immunosensors based on layer-by-layer self-assembled carbon nanotubes and biomolecules

Miao Lu, Dongjin Lee, Wei Xue, Tianhong Cui

Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.

Original language	English (US)
Pages (from-to)	280-285
Number of pages	6
Journal	Sensors and Actuators, A: Physical
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/j.sna.2008.12.021
State	Published - Mar 25 2009

Keywords

Immunosensor
Layer-by-layer (LbL) self-assembly
MEMS
Single-walled carbon nanotube (SWNT)

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10.1016/j.sna.2008.12.021

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abstract = "A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.",

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AU - Lu, Miao

AU - Lee, Dongjin

AU - Xue, Wei

AU - Cui, Tianhong

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Y1 - 2009/3/25

N2 - A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.

AB - A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.

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KW - MEMS

KW - Single-walled carbon nanotube (SWNT)

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