Abstract
A low-cost, flexible, and disposable immunosensor is presented in this paper. The single-walled carbon nanotubes (SWNTs) and biomolecules are self-assembled between two micro-patterned electrodes. The immuno-chip acts as a platform of a horseradish peroxidase (HRP) labeled sandwiched Enzyme-Linked ImmunoSorbent Assay (ELISA). The pH change induced by the biochemical reactions influences the electrical conductance of SWNT. A detection resolution of 0.4 ng/ml (2.5 pM) for normal rabbit immunoglobulin G (IgG) is demonstrated. The new fabrication technique and the HRP labeled detection protocol can be extended to the recognition of other antigens for critical applications to clinical diagnosis, food toxin detection, and environment monitoring.
Original language | English (US) |
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Pages (from-to) | 280-285 |
Number of pages | 6 |
Journal | Sensors and Actuators, A: Physical |
Volume | 150 |
Issue number | 2 |
DOIs | |
State | Published - Mar 25 2009 |
Bibliographical note
Funding Information:This work is partially supported by DARPA MEMS/NEMS Fundamental Research Program through the MF3 Center.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
Keywords
- Immunosensor
- Layer-by-layer (LbL) self-assembly
- MEMS
- Single-walled carbon nanotube (SWNT)