Flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in mosquito cells

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Abstract

Conditions for flow cytometric evaluation of the intracellular bacterium, Wolbachia pipientis, in infected mosquito cells are described. This approach will streamline investigation of Wolbachia's interactions with host cells and facilitate identification of culture conditions that select for Wolbachia-infected cells.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalJournal of Microbiological Methods
Volume107
DOIs
StatePublished - Oct 1 2014

Bibliographical note

Funding Information:
This work was supported by NIH grant AI 081322 and by the University of Minnesota Agricultural Experiment Station, St. Paul, MN . I thank Drs. T.J. Kurtti and U.G. Munderloh for helpful advice.

Publisher Copyright:
© 2014 Elsevier B.V.

Keywords

  • Alphaproteobacteria
  • Flow cytometry
  • Intracellular microorganism
  • Mosquito cell line
  • Wolbachia

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