Fungal burden in the cerebrospinal fluid is an important determinant of mortality in cryptococcal meningitis, but its use in aiding clinical decision making is hampered by the time involved to perform quantitative cultures. Here, we demonstrate the potential of flow cytometry as a novel and rapid technique to address this issue.
Bibliographical noteFunding Information:
We thank Rosie Burton, Gavin van Wyk, and Anthony Williams along with the clinical, laboratory, and administrative staff of the Provincial Government of the Western Cape Department of Health for their support of the study and assistance. We declare no conflicts of interest. The funders had no role in the study design, data collection, data analysis, data interpretation, or writing of this report. The opinions, findings, and conclusions expressed in this paper reflect those of the authors alone. National Research Foundation of South Africa provided funding to Robert J. Wilkinson under grant number 96841. European Union provided funding to Robert J. Wilkinson under grant numbers FP7-PEOPLE-2011- IRSES and FP7-HEALTH-F3-2012-305578. Wellcome Trust provided funding to James E. Scriven under grant number 094013/B/10/Z. Wellcome Trust provided funding to Graeme Meintjes under grant number 098316. Wellcome Trust provided funding to Robert J. Wilkinson under grant number 104803. Wellcome Trust provided funding to Britta C. Urban under grant number 079082. Medical Research Council (MRC) provided funding to Robert J. Wilkinson under grant number U1175.02.002.00014.01. DH | National Institute for Health Research (NIHR) provided funding to David R. Boulware under grant number U01AI089244. South African Medical Research Council (SAMRC) provided funding to Graeme Meintjes under grant number 64787.