Fluorescence detection of cationic amyloid fibrils in human semen

David Easterhoff; John T.M. Dimaio; Wathsala Liyanage; Chi Wen Lo; Woori Bae; Todd M. Doran; Alan Smrcka; Bradley L. Nilsson; Stephen Dewhurst

doi:10.1016/j.bmcl.2013.06.097

Fluorescence detection of cationic amyloid fibrils in human semen

David Easterhoff, John T.M. Dimaio, Wathsala Liyanage, Chi Wen Lo, Woori Bae, Todd M. Doran, Alan Smrcka, Bradley L. Nilsson, Stephen Dewhurst

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.

Original language	English (US)
Pages (from-to)	5199-5202
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	23
Issue number	18
DOIs	https://doi.org/10.1016/j.bmcl.2013.06.097
State	Published - Sep 15 2013

Bibliographical note

Funding Information:
Supported by NIH grants T32AI049815 (DE), R21AI094511 . This publication was also supported in part by the University of Rochester Center for AIDS Research (NIH P30AI078498 ) and by a Drug Development pilot award from the University of Rochester School of Medicine & Dentistry .

Keywords

Amyloid
Fluorescence
SEVI
Semen
Thioflavin T

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title = "Fluorescence detection of cationic amyloid fibrils in human semen",

abstract = "Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.",

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note = "Funding Information: Supported by NIH grants T32AI049815 (DE), R21AI094511 . This publication was also supported in part by the University of Rochester Center for AIDS Research (NIH P30AI078498 ) and by a Drug Development pilot award from the University of Rochester School of Medicine & Dentistry . ",

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AU - Easterhoff, David

AU - Dimaio, John T.M.

AU - Liyanage, Wathsala

AU - Lo, Chi Wen

AU - Bae, Woori

AU - Doran, Todd M.

AU - Smrcka, Alan

AU - Nilsson, Bradley L.

AU - Dewhurst, Stephen

N1 - Funding Information: Supported by NIH grants T32AI049815 (DE), R21AI094511 . This publication was also supported in part by the University of Rochester Center for AIDS Research (NIH P30AI078498 ) and by a Drug Development pilot award from the University of Rochester School of Medicine & Dentistry .

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Y1 - 2013/9/15

N2 - Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.

AB - Cationic amyloid fibrils, including the Semen Enhancer of Virus Infection (SEVI), have recently been described in human semen. Simple methods for quantitating these fibrils are needed to improve our understanding of their biological function. We performed high-throughput screening to identify molecules that bind SEVI, and identified a small molecule (8E2), that fluoresced brightly in the presence of SEVI and other cationic fibrils. 8E2 bound SEVI with almost 40-fold greater affinity than thioflavin-T, and could efficiently detect high molecular weight fibrils in human seminal fluid.

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KW - Fluorescence

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KW - Semen

KW - Thioflavin T

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Fluorescence detection of cationic amyloid fibrils in human semen

Abstract

Bibliographical note

Keywords

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