Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells

Nur P. Damayanti; Laurie L. Parker; Joseph M K Irudayaraj

doi:10.1002/anie.201209303

Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells

Nur P. Damayanti, Laurie L. Parker, Joseph M K Irudayaraj

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Better living through biochemistry: Phosphorylation of a Cy5-labeled Abl kinase peptide biosensor resulted in an extension of fluorescence lifetime in live cells. Time-correlated single photon counting fluorescence lifetime imaging (FLIM) enabled excellent signal-to-noise to visualize the subcellular patterns of this sensor (see picture). This strategy should be generalizable to other peptide-based kinase substrates for imaging of kinase activity in single cells.

Original language	English (US)
Pages (from-to)	3931-3934
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	14
DOIs	https://doi.org/10.1002/anie.201209303
State	Published - Apr 2 2013

Keywords

Abl kinase
biosensors
fluorescence lifetime imaging microscopy
fluorescent probes
inhibitors

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells

Abstract

Keywords

UN SDGs

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