TY - JOUR
T1 - Fluorescence microscopy.
AU - Herman, Brian
PY - 2002/5
Y1 - 2002/5
N2 - Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom.
AB - Absorption of energy as light by some molecules and emission of energy as fluorescence can occur only at certain wavelengths, which are characteristic for a given molecule (fluorophore). Fluorescence microscopes are equipped to observe the fluorescence of one or more specific fluorophores to localize specific molecules and analyzed cellular structures. This unit discusses the optics and other components of the fluorescence microscope, their functions and adjustments, and the digital darkroom.
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U2 - 10.1002/0471142735.im2102s48
DO - 10.1002/0471142735.im2102s48
M3 - Article
C2 - 18432880
AN - SCOPUS:43549085272
VL - Chapter 21
SP - Unit 21.2
JO - Current protocols in immunology / edited by John E. Coligan ... [et al.]
JF - Current protocols in immunology / edited by John E. Coligan ... [et al.]
SN - 1934-3671
ER -