Fluorescent Pyrene Derivative of Concanavalin A: Preparation and Spectroscopic Characterization

Brian A. Herman; Salvador M. Fernandez

doi:10.1021/bi00257a004

Fluorescent Pyrene Derivative of Concanavalin A: Preparation and Spectroscopic Characterization

Brian A. Herman, Salvador M. Fernandez

Biomedical Engineering

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Abstract

The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.

Original language	English (US)
Pages (from-to)	3271-3275
Number of pages	5
Journal	Biochemistry
Volume	21
Issue number	14
DOIs	https://doi.org/10.1021/bi00257a004
State	Published - Jul 1982

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title = "Fluorescent Pyrene Derivative of Concanavalin A: Preparation and Spectroscopic Characterization",

abstract = "The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.",

author = "Herman, {Brian A.} and Fernandez, {Salvador M.}",

year = "1982",

month = jul,

doi = "10.1021/bi00257a004",

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volume = "21",

pages = "3271--3275",

journal = "Biochemistry",

issn = "0006-2960",

publisher = "American Chemical Society",

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T1 - Fluorescent Pyrene Derivative of Concanavalin A

T2 - Preparation and Spectroscopic Characterization

AU - Herman, Brian A.

AU - Fernandez, Salvador M.

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N2 - The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.

AB - The preparation and characterization of a fluorescent 1-pyreneacrylate-concanavalin A conjugate are described. Excitation, emission, and polarization spectra are presented as well as quantum yield and fluorescence lifetimes. Perrin plots examining the rotational behavior of the pyrene-Con A complex show good agreement with those previously published for other fluorophore-Con A conjugates. The suitability of this flurorphore as a donor for resonance energy transfer studies is discussed.

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ER -

Fluorescent Pyrene Derivative of Concanavalin A: Preparation and Spectroscopic Characterization

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