Previously we found that porous microparticulate zirconium oxide strongly adsorbs fluoride from aqueous solutions. The adsorption is due in large part to a Lewis acid-base interaction between coordination sites on the zirconium oxide surface and fluoride. The resulting complex is very stable relative to many other species and therefore fluoride is hard to displace. The chromatographic properties of small molecules and proteins on zirconium oxide particles when fluoride is present in the mobile phase is investigated in this work. In the presence of fluoride, zirconium oxide is a very biocompatible adsorbent, the selectivities of which are analogous to those of calcium hydroxyapatite. The effect of fluoride is very reproducible even after the adsorbed fluoride is stripped by strong base and regenerated in fluoride buffer.
Bibliographical noteFunding Information:
The authorsw ould like to thank Eric FunkenbuschD, ouglasH anggi and Tom Weber for their helpful discussionsa nd Don Hagen for his encouragemenJt..A .B. acknowledgefsi nancial support from SpecialtyA dhesivesa nd ChemicalsD ivision and the LeadingE dgeA cademicP rograma t 3M. This work wass upportedin part by grantsf rom the 3M Companya nd the Institutef or AdvancedS tudiesi n Bioprocess Technology.