Folate-receptor-mediated delivery of InP quantum dots for bioimaging using confocal and two-photon microscopy

Dhruba J. Bharali, Derrick W. Lucey, Harishankar Jayakumar, Haridas E. Pudavar, Paras N. Prasad

Research output: Contribution to journalArticlepeer-review

Abstract

A novel method for the synthesis of highly monodispersed hydrophillic InP-ZnS nanocrystals and their use as luminescence probes for live cell imaging is reported. Hydrophobic InP-ZnS nanocrystals are prepared by a new method that yields high-quality, luminescent core-shell nanocrystals within 6-8 h of total reaction time. Then by carefully manipulating the surface of these passivated nanocrystals, aqueous dispersions of folate-conjugated nanocrystals (folate-QDs) with high photostability are prepared. By use of confocal microscopy, we demonstrate the receptor-mediated delivery of folic acid conjugated quantum dots into folate-receptor-positive cell lines such as KB cells. These folate-QDs tend to accumulate in multivescicular bodies of KB cells after 6 h of incubation. Receptor-mediated delivery was confirmed by comparison with the uptake of these particles in folate-receptor-negative cell lines such as A549. Efficient two-photon excitation of these particles and two-photon imaging using these particles are also demonstrated. The use of these InP-ZnS nanoparticles and their efficient two-photon excitation can be potentially useful for deep tissue imaging for future in vivo studies.

Original languageEnglish (US)
Pages (from-to)11364-11371
Number of pages8
JournalJournal of the American Chemical Society
Volume127
Issue number32
DOIs
StatePublished - Aug 17 2005
Externally publishedYes

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