Foliar- and fruit-applied strontium as a tracer for calcium transport in apple trees

Application of calcium (Ca) sprays is a recommended practice to reduce the incidence of Ca-related disorders such as bitter pit in apple (Malus x domestica), but effectiveness of sprays to increase Ca concentrations in the fruit is not always consistent. Strontium (Sr) has been used as a Ca analog to evaluate Ca transport processes and distribution in plants. A field study was conducted using foliar- and fruit-applied Sr as a tracer for Ca transport in 20-year-old 'Honeycrisp' apple trees on Malling.26 (M.26) rootstock. The objectives of this study were to 1) measure the amount of Sr translocation from leaves to fruit, 2) determine the effectiveness of eight sprays applied over the growing season vs. four late-season sprays on increasing Sr concentrations in leaves and fruit, and 3) evaluate the effect of an experimental adjuvant consisting of alkyl-polysaccharides and monosaccharides on spray efficacy. Seven treatments were tested, which included a control and six Sr treatments applied in various combinations with or without an adjuvant. Trees were sprayed four or eight times during the growing season, either directly to leaves and fruit or to leaves only (fruit covered during application). Spray treatments did not significantly affect total fruit fresh or dry weight. Although some discrimination between Ca and Sr was detected, the similar distribution of Ca and Sr in fruit tissue of control treatments suggested that Sr is a suitable tracer for Ca. Based on the covered vs. uncovered fruit treatments, about 11% to 17% of the Sr in the fruit came from Sr applied directly to the leaves. Eight spray applications over the growing season more than doubled both the concentration and content of fruit Sr compared with four late season sprays. The tested adjuvant doubled Sr absorption by and translocation to fruit compared with not using an adjuvant. Assuming similar transport for Ca and Sr, and adjusting for the atomic weight of Ca relative to Sr, the maximum increase in fruit Ca concentration at harvest from foliar and fruit applications (eight sprays with adjuvant and uncovered fruit) would have been as follows: core = 78 mg·kg^-1; flesh = 35 mg·kg^-1; peel = 195 mg·kg^-1; entire fruit = 67 mg·kg^-1. In addition to being an underused tool for studying Ca transport patterns, the results also suggest that use of Sr may be a novel technique for testing the efficacy of various adjuvants used to enhance uptake and transport of Ca in leaves and fruit.