Formation and analysis of heterocyclic aromatic amine-DNA adducts in vitro and in vivo

Robert J. Turesky; Paul Vouros

doi:10.1016/j.jchromb.2003.10.053

Formation and analysis of heterocyclic aromatic amine-DNA adducts in vitro and in vivo

Robert J. Turesky, Paul Vouros

Medicinal Chemistry

Research output: Contribution to journal › Review article › peer-review

86 Scopus citations

Abstract

The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the ³²P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.

Original language	English (US)
Pages (from-to)	155-166
Number of pages	12
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	802
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2003.10.053
State	Published - Mar 25 2004

Keywords

DNA
Heterocyclic aromatic amine-DNA adducts
Reviews

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abstract = "The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.",

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AB - The detection and quantification of heterocyclic aromatic amine (HAA)-DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA-DNA adducts are the 32P-postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC-ESI-MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA-DNA adduct in vitro and vivo are discussed.

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