FTIR study of conformational substates in the CO adduct of cytochrome c oxidase from Rhodobacter sphaeroides

David M. Mitchell, Joachim D. Müller, Robert B. Gennis, G. Ulrich Nienhaus

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22 Scopus citations

Abstract

Fourier transform infrared (FTIR) spectroscopy of cytochrome c oxidase from Rhodobacter sphaeroides reveals multiple CO stretch bands that are associated with different conformational substates of the enzyme. Here we report the temperature dependence of the infrared bands for the CO bound to the Fe(a3) heme iron and to Cu(B). We have also studied the kinetics of ligand return from Fe(a3) to Cu(B) using temperature derivative spectroscopy (TDS). Two classes of substates (α/β) can be distinguished from their different properties with regard to the width of the IR band, the temperature dependence of the peak position, and the peak of the enthalpy distribution. The pronounced temperature dependence of the stretch frequencies in the β conformation and the lack thereof in the α conformation implies very different dynamic behavior in the active site and reflects structural differences between the two conformations, most likely a shift of the position of Cu(B) in response to a change in its stereochemical environment. Similar conformational changes will be necessary during the catalytic cycle of the enzyme when dioxygen is bound in the active site.

Original languageEnglish (US)
Pages (from-to)16782-16788
Number of pages7
JournalBiochemistry
Volume35
Issue number51
DOIs
StatePublished - Dec 24 1996

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