Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Yusuke Minato; Fereshteh Shahcheraghi; Wakano Ogawa; Teruo Kuroda; Tomofusa Tsuchiya

doi:10.1248/bpb.31.516

Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

Yusuke Minato, Fereshteh Shahcheraghi, Wakano Ogawa, Teruo Kuroda, Tomofusa Tsuchiya

Microbiology

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28 Scopus citations

Abstract

We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.

Original language	English (US)
Pages (from-to)	516-519
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	31
Issue number	3
DOIs	https://doi.org/10.1248/bpb.31.516
State	Published - Mar 2008

Keywords

Drug resistance
Multidrug efflux pump
Serratia marcescens
SsmE

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abstract = "We cloned a gene responsible for multidrug resistance from chromosomal DNA of Serratia marcescens, and determined the nucleotide sequence. We designated the gene as ssmE. The deduced amino acid sequence of SsmE showed high similarity with the small multidrug resistance (SMR)-type multidrug efflux pumps. Cells of Escherichia coli KAM32, a drug hyper-susceptible mutant, transformed with a plasmid pESM437 carrying the ssmE gene showed elevated minimum inhibitory concentrations of structurally unrelated antimicrobial agents. E. coli KAM32/pESM437 showed elevated energy dependent efflux of ethidium. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed that ssmE was expressed in cells of S. marcescens.",

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AU - Shahcheraghi, Fereshteh

AU - Ogawa, Wakano

AU - Kuroda, Teruo

AU - Tsuchiya, Tomofusa

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Functional gene cloning and characterization of the SsmE multidrug efflux pump from Serratia marcescens

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