The diaphragm muscle must be able to generate sufficient forces to accomplish a range of ventilatory and non-ventilatory behaviors throughout life. Measurements of transdiaphragmatic pressure (Pdi) can be conducted during eupnea, hypoxia (10 % O2)-hypercapnia (5 % CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths (i.e., sighs), sustained airway or tracheal occlusion, and maximal efforts elicited via bilateral phrenic nerve stimulation, representing the full range of motor behaviors available by the diaphragm muscle. We provide detailed methods on the in vivo measurements of Pdi in mice.
|Original language||English (US)|
|Title of host publication||Methods in Molecular Biology|
|Publisher||Humana Press Inc.|
|Number of pages||11|
|State||Published - Aug 6 2016|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
Any procedures conducted on animals in the development of these methods were conducted following institutional protocols and animal care guidelines, in compliance with National Institute of Health Guidelines. This work was supported by grants from National Institute of Health R01-AG-044615 and R01-HL-096750 (CBM and GCS), T32-HL105355 (SMG), and the Mayo Clinic.
- Diaphragm muscle
- Muscle force
- Non-ventilatory behavior
- Phrenic nerve
- Ventilatory behavior