The type 5 G protein Β subunit (GΒ5) can form complexes with members of the regulator of G protein signaling 7 (RGS7) family, but its relevance to neuronal G protein signaling is unclear. We found that mouse RGS7-GΒ5 complexes bound to G protein-gated potassium channels and facilitated their functional coupling to GABA B receptors in neurons. Our findings identify a compartmentalization mechanism that is critical for ensuring high temporal resolution of neuronal G protein signaling.
Bibliographical noteFunding Information:
We thank J. Chen for providing the Gβ5−/− mice, W. Simonds for the gift of antibodies to Gβ5 and RGS7 and M. Parent for assistance with the hippocampal slicing technique. This work was supported by US National Institutes of Health grants DA021743 (K.A.M.), DA026405 (K.A.M.), EY018139 (K.A.M.), MH061933 (K.W.), DA011806 (K.W.), DA019666 (M.J.T.) and MH078291 (J.C.-S.), a McKnight Land-Grant Award (K.A.M), and an award from the Whitehall Foundation (M.J.T.).