GABAA receptor β1, β2, and β3 subunits: comparisons in DBA/2J and C57BL/6J mice

Ganesan L. Kamatchi; Paulo Kofuji; Jia Bei Wang; John C R Fernando; Zhifang Liu; Jeevan R. Mathura; David R. Burt

doi:10.1016/0167-4781(95)00009-6

GABA_A receptor β₁, β₂, and β₃ subunits: comparisons in DBA/2J and C57BL/6J mice

Ganesan L. Kamatchi, Paulo Kofuji, Jia Bei Wang, John C R Fernando, Zhifang Liu, Jeevan R. Mathura, David R. Burt

Neuroscience

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

GABA_A receptors link binding of GABA (γ-aminobutyric acid) to inhibitory chloride flux in the brain. They are the site of action of several important classes of drugs, and have been implicated in animal models of epilepsy and in the actions of alcohol. We compare the sequence and expression of the β1, β2 and β3 subunits of GABA_A receptors in two inbred strains of mice, DBA/2J and C57BL/6J, which differ markedly in seizure susceptibility and in a variety of behaviors related to alcohol. Only the β3 3 subunit had strain differences in cDNA nucleotide sequence, which did not affect amino acid sequence but which did create restriction fragment length polymorphisms (RFLPs) potentially useful in gene mapping. We have also tested mouse β1 and β2 subunits for internal alternative splicing, detecting none.

Original language	English (US)
Pages (from-to)	134-142
Number of pages	9
Journal	BBA - Gene Structure and Expression
Volume	1261
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(95)00009-6
State	Published - Mar 14 1995

Bibliographical note

Funding Information:
Drs. Eric Barnard and Mark Darlison are thanked for the gift of a bovine /31 subunit cDNA, Drs. Stephen J. Moss and Richard L. Huganir for the gift of some primers, and David K. Overman, Jarsie Weeks and Richard Douglas for technical assistance. This work was supported by USPHS grants NS25525 and AA07559 to D.R.B.

Keywords

(Mouse)
Alcohol
Epilepsy
GABA/benzodiazepine receptor
Gene mapping
RFLP
cDNA
γ-Aminobutyric acid

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title = "GABAA receptor β1, β2, and β3 subunits: comparisons in DBA/2J and C57BL/6J mice",

abstract = "GABAA receptors link binding of GABA (γ-aminobutyric acid) to inhibitory chloride flux in the brain. They are the site of action of several important classes of drugs, and have been implicated in animal models of epilepsy and in the actions of alcohol. We compare the sequence and expression of the β1, β2 and β3 subunits of GABAA receptors in two inbred strains of mice, DBA/2J and C57BL/6J, which differ markedly in seizure susceptibility and in a variety of behaviors related to alcohol. Only the β3 3 subunit had strain differences in cDNA nucleotide sequence, which did not affect amino acid sequence but which did create restriction fragment length polymorphisms (RFLPs) potentially useful in gene mapping. We have also tested mouse β1 and β2 subunits for internal alternative splicing, detecting none.",

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