Gene expression of type I phospholipase A2 in pancreatic beta cells. Regulation of mRNA levels by starvation or glucose excess

S. Metz, D. Holmes, R. P. Robertson, W. Leitner, B. Draznin

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats: they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150±6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second-phase release = 70±11%). These studies uniquely demonstrate that type I phospholipase A2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.

Original languageEnglish (US)
Pages (from-to)110-112
Number of pages3
JournalFEBS Letters
Volume295
Issue number1-3
DOIs
StatePublished - Dec 16 1991

Keywords

  • Beta cell
  • Fasting
  • Glucose
  • Insulin
  • Pancreatic islet
  • Phospholipase

Fingerprint Dive into the research topics of 'Gene expression of type I phospholipase A<sub>2</sub> in pancreatic beta cells. Regulation of mRNA levels by starvation or glucose excess'. Together they form a unique fingerprint.

Cite this