Gene expression signatures from three genetically separable resistance gene signaling pathways for downy mildew resistance

Thomas Eulgem, Victor J. Weigman, Hur Song Chang, John M. McDowell, Eric B. Holub, Jane Glazebrook, Tong Zhu, Jeffery L. Dangl

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Resistance gene-dependent disease resistance to pathogenic microorganisms is mediated by genetically separable regulatory pathways. Using the GeneChip Arabidopsis genome array, we compared the expression profiles of approximately 8,000 Arabidopsis genes following activation of three RPP genes directed against the pathogenic oomycete Peronospora parasitica. Judicious choice of P. parasitica isolates and loss of resistance plant mutants allowed us to compare the responses controlled by three genetically distinct resistance gene-mediated signaling pathways. We found that all three pathways can converge, leading to up-regulation of common sets of target genes. At least two temporal patterns of gene activation are triggered by two of the pathways examined. Many genes defined by their early and transient increases in expression encode proteins that execute defense biochemistry, while genes exhibiting a sustained or delayed expression increase predominantly encode putative signaling proteins. Previously defined and novel sequence motifs were found to be enriched in the promoters of genes coregulated by the local defense-signaling network. These putative promoter elements may operate downstream from signal convergence points.

Original languageEnglish (US)
Pages (from-to)1129-1144
Number of pages16
JournalPlant physiology
Volume135
Issue number2
DOIs
StatePublished - Jun 2004

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