Facilitation of solid organ and cell transplantation depends on metabolic and immunologic factors that can be manipulated ex vivo and in vivo using gene transfer technology. Vectors have been developed which can optimally transfer relevant genes to various tissues and organs. Interventions aimed at promoting tissue preservation before transplantation, prevention of oxidative stress and immunological rejection have recently become attractive options using viral and nonviral gene delivery vehicles. Further understanding of the mechanisms involved in tolerance induction as well as the facilitation of xenogeneic engraftment have made possible a variety of avenues that can be exploited using gene transfer technology.
Bibliographical noteFunding Information:
The authors would like to acknowledge the insightful comments of Dr Adrian Morelli on this review. This work was supported in part by Public Health Services grant DK 44935 to PDR and AI 41011 to AWT. NG is a recipient of a postdoctoral fellowship award from the Juvenile Diabetes Foundation International as well as a prize from the Fonds pour la formation de chercheurs et a l’aide a la recherche (Fonds FCAR) from the provincial government of Quebec, Canada.
- Gene therapy