Gene transfer of calcium-binding proteins into adult cardiac myocytes

Brian R Thompson, Houda Cohen, Addeli Bez Batti Angulski, Joseph M Metzger

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Heart failure is the leading cause of combined morbidity and mortality in the USA with 50% of cases being diastolic heart failure. Diastolic heart failure results from poor myocardial relaxation and inadequate filling of the left ventricular chamber caused in part by calcium-handling dysregulation. In this chapter we describe methods to investigate new approaches of novel human Ca 2+ binding protein motifs to restore normal Ca 2+ handling function to diseased myocardium. Gene transfer of parvalbumin into adult cardiac myocytes has been studied as a potential therapeutic, specifically as a strategic Ca 2+ buffer to correct cardiac mechanical dysfunction in disease. This chapter provides protocols for studying wild-type parvalbumin isoforms and parvalbumins with strategically designed EF-hand motifs in adult cardiac myocytes via acute adenoviral gene transfer. These protocols have been used extensively to optimize parvalbumin function as a potential therapeutic for failing heart muscle.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages187-205
Number of pages19
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1929
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by funds from NIH.

Keywords

  • Adult cardiac myocyte
  • Calcium
  • Calcium imaging
  • Contractility
  • Gene transfer
  • Parvalbumin

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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