Generation of a nested series of interstitial deletions in yeast artificial chromosomes carrying human DNA

Colin Campbell, Rajiv Gulati, Asit K. Nandi, Kimberly Floy, Philip Hieter, Raju S. Kucherlapati

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We have generated a nested series of interstitial deletions in a fragment of human X chromosome-derived DNA cloned into a yeast artificial chromosome (YAC) vector. A yeast strain carrying the YAC was transformed with a linear recombination substrate containing at one end a sequence that is uniquely represented on the YAC and at the other end a truncated long interspersed repetitive element (LINE 1, or L1). Homologous recombination between the YAC and the input DNA resulted in a nested series of interstitial deletions, the largest of which was 500 kilobases. In combination with terminal deletions that can be generated through homologous recombination, the interstitial deletions are useful for mapping and studying gene structure-function relationships.

Original languageEnglish (US)
Pages (from-to)5744-5748
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number13
DOIs
StatePublished - Jul 1 1991

Keywords

  • Factor IX gene
  • Homologous recombination
  • Repetitive elements

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