TY - JOUR
T1 - Generation of guanine-thymine cross-links in human cells by one-electron oxidation mechanisms
AU - Madugundu, Guru S.
AU - Wagner, J. Richard
AU - Cadet, Jean
AU - Kropachev, Konstantin
AU - Yun, Byeong Hwa
AU - Geacintov, Nicholas E.
AU - Shafirovich, Vladimir
PY - 2013/7/15
Y1 - 2013/7/15
N2 - The one-electron oxidation of cellular DNA in cultured human HeLa cells initiated by intense nanosecond 266 nm laser pulse irradiation produces cross-links between guanine and thymine bases (G*-T*), characterized by a covalent bond between C8 guanine (G*) and N3 thymine (T*) atoms. The DNA lesions were quantified by isotope dilution LC-MS/MS methods in the multiple reaction-monitoring mode using isotopically labeled [ 15N, 13C]-nucleotides as internal standards. Among several known pyrimidine and 8-oxo-7,8-dihydroguanine lesions, the G*-T* cross-linked lesions were detected at levels of ∼0.21 and 1.19 d(G*-T*) lesions per 106 DNA bases at laser intensities of 50 and 280 mJ/cm2/pulse, respectively.
AB - The one-electron oxidation of cellular DNA in cultured human HeLa cells initiated by intense nanosecond 266 nm laser pulse irradiation produces cross-links between guanine and thymine bases (G*-T*), characterized by a covalent bond between C8 guanine (G*) and N3 thymine (T*) atoms. The DNA lesions were quantified by isotope dilution LC-MS/MS methods in the multiple reaction-monitoring mode using isotopically labeled [ 15N, 13C]-nucleotides as internal standards. Among several known pyrimidine and 8-oxo-7,8-dihydroguanine lesions, the G*-T* cross-linked lesions were detected at levels of ∼0.21 and 1.19 d(G*-T*) lesions per 106 DNA bases at laser intensities of 50 and 280 mJ/cm2/pulse, respectively.
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U2 - 10.1021/tx400158g
DO - 10.1021/tx400158g
M3 - Article
C2 - 23734842
AN - SCOPUS:84880280825
SN - 0893-228X
VL - 26
SP - 1031
EP - 1033
JO - Chemical research in toxicology
JF - Chemical research in toxicology
IS - 7
ER -