TY - JOUR
T1 - Generation of monoclonal antibodies to murine IL-1β and demonstration of IL-1 in vivo
AU - Hogquist, Kristin A.
AU - Nett, Michelle A.
AU - Sheehan, Kathleen C F
AU - Pendleton, Kimberly D.
AU - Schreiber, Robert D.
AU - Chaplin, David D.
PY - 1991/3/1
Y1 - 1991/3/1
N2 - The role of murine IL-1β in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1β and their application to a characterization of the murine IL-1β protein. Immunization of either hamsters or rabbits with the recombinant mature form of murine IL-1β emulsified in CFA elicited antisera and hamster mAb that only recognized denatured IL-1β. In contrast, immunization with rIL-1β adsorbed to alum resulted in the generation of neutralizing and immunoprecipitating rabbit and hamster antisera and hamster mAb. All of the mAb recognize both the pro-form of IL-1β and the mature bioactive form produced by cultures of murine peritoneal macrophages. Using these antibodies, we demonstrate that approximately half of the IL-1 activity present in supernatants of LPS-treated cultured mouse macrophages is composed of IL-1β. Additionally, IL-1β as well as IL-1α can be detected in the plasma of LPS-treated mice. These studies, therefore, demonstrate the production of IL-1β both in vitro and in vivo.
AB - The role of murine IL-1β in vitro and in vivo has not been defined. We describe here the production of neutralizing and immunoprecipitating mAb and polyclonal antibodies specific for murine IL-1β and their application to a characterization of the murine IL-1β protein. Immunization of either hamsters or rabbits with the recombinant mature form of murine IL-1β emulsified in CFA elicited antisera and hamster mAb that only recognized denatured IL-1β. In contrast, immunization with rIL-1β adsorbed to alum resulted in the generation of neutralizing and immunoprecipitating rabbit and hamster antisera and hamster mAb. All of the mAb recognize both the pro-form of IL-1β and the mature bioactive form produced by cultures of murine peritoneal macrophages. Using these antibodies, we demonstrate that approximately half of the IL-1 activity present in supernatants of LPS-treated cultured mouse macrophages is composed of IL-1β. Additionally, IL-1β as well as IL-1α can be detected in the plasma of LPS-treated mice. These studies, therefore, demonstrate the production of IL-1β both in vitro and in vivo.
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M3 - Article
C2 - 1993843
AN - SCOPUS:0026073378
SN - 0022-1767
VL - 146
SP - 1534
EP - 1540
JO - Journal of Immunology
JF - Journal of Immunology
IS - 5
ER -