Generation of new enzymes via covalent modification of existing proteins

Dongfeng Qi; Cheng Min Tann; Dietmar Haring; Mark D Distefano

doi:10.1021/cr000059o

Generation of new enzymes via covalent modification of existing proteins

Dongfeng Qi, Cheng Min Tann, Dietmar Haring, Mark D Distefano

Chemistry (Twin Cities)

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246 Scopus citations

Abstract

The different approaches for chemically modifying enzymes and proteins to obtain catalysts with either modified or completely new catalytic activities were described. With the advent of site directed mutagenesis, it is possible to introduce cysteine residues that can be introduced at any desired location and subsequently functionalized with a wide range reagents. Significant rate of catalytic acceleration was obtained in some cases.

Original language	English (US)
Pages (from-to)	3081-3111
Number of pages	31
Journal	Chemical Reviews
Volume	101
Issue number	10
DOIs	https://doi.org/10.1021/cr000059o
State	Published - Oct 1 2001

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title = "Generation of new enzymes via covalent modification of existing proteins",

abstract = "The different approaches for chemically modifying enzymes and proteins to obtain catalysts with either modified or completely new catalytic activities were described. With the advent of site directed mutagenesis, it is possible to introduce cysteine residues that can be introduced at any desired location and subsequently functionalized with a wide range reagents. Significant rate of catalytic acceleration was obtained in some cases.",

author = "Dongfeng Qi and Tann, {Cheng Min} and Dietmar Haring and Distefano, {Mark D}",

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AU - Distefano, Mark D

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Generation of new enzymes via covalent modification of existing proteins

Abstract

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