Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat

C. D. Otto, S. F. Kianian, E. M. Elias, R. W. Stack, L. R. Joppa

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91 Scopus citations

Abstract

The devastating effect of Fusarium head blight (FHB) caused by Fusarium graminearum has led to significant financial losses across the Upper Midwest of the USA. These losses have spurred the need for research in biological, chemical, and genetic control methods for this disease. To date, most of the research on FHB resistance has concentrated on hexaploid wheat (Triticum aestivum L.) lines originating from China. Other sources of resistance to FHB would be desirable. One other source of resistance for both hexaploid wheat and tetraploid durum wheat (T. turgidum L. var. durum) is the wild tetraploid, T. turgidum L. var. dicoccoides (T. dicoccoides). Previous analysis of the 'Langdon'-T. dicoccoides chromosome substitution lines, LDN(Dic), indicated that the chromosome 3A substitution line expresses moderate levels of resistance to FHB. LDN(Dic-3A) recombinant inbred chromosome lines (RICL) were used to generate a linkage map of chromosome 3A with 19 molecular markers spanning a distance of 155.2 cM. The individual RICL and controls were screened for their FHB phenotypein two greenhouse seasons. Analysis of 83 RICL identified a single major quantitative trait locus, Qfhs.ndsu-3AS, that explains 37% of the phenotypic or 55% of the genetic variation for FHB resistance. A microsatellite locus, Xgwm2, is tightly linked to the highest point of the QTL peak. A region of the LDN (Dic-3A) chromosome associated with the QTL for FHB resistance encompasses a 29.3 cM region from Xmwg14 to Xbcd828.

Original languageEnglish (US)
Pages (from-to)625-632
Number of pages8
JournalPlant molecular biology
Volume48
Issue number5-6
DOIs
StatePublished - 2002

Bibliographical note

Funding Information:
We would like to thank Dr Jennifer Mitchell-Fetch, Mary Johnshoy, Dr. José González-Hernández, Jana Hansen, and Justin Hegstad for all their assistance in making this paper possible. Special thanks go to Erik Doehler for his work with the RGA primers. His help was made possible by a funding from the NSF-EPSCoR Science Bound Project. We would also like to thank the anonymous reviewers for their suggestions in improving this manuscript. We gratefully acknowledge grant support from the USDA-ARS, U.S. Wheat and Barley Scab Initiative (59-0790-9-048) and the North Dakota Wheat Commission to S.F.K.

Keywords

  • Durum wheat
  • Fusarium head blight
  • QTL
  • Recombinant inbred chromosome lines
  • Triticum dicoccoides

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