TY - JOUR
T1 - Genetic influences on brain gene expression in rats selected for tameness and aggression
AU - Heyne, Henrike O.
AU - Lautenschläger, Susann
AU - Nelson, Ronald
AU - Besnier, François
AU - Rotival, maxime
AU - Cagan, Alexander
AU - Kozhemyakina, Rimma
AU - Plyusnina, Irina Z.
AU - Trut, Lyudmila
AU - Carlborg, Örjan
AU - Petretto, Enrico
AU - Kruglyak, Leonid
AU - Pääbo, Svante
AU - Schöneberg, Torsten
AU - Albert, Frank W.
N1 - Publisher Copyright:
© 2014 by the Genetics Society of America.
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Interindividual differences in many behaviors are partly due to genetic differences, but the identification of the genes and variants that influence behavior remains challenging. Here, we studied an F2 intercross of two outbred lines of rats selected for tame and aggressive behavior toward humans for .64 generations. By using a mapping approach that is able to identify genetic loci segregating within the lines, we identified four times more loci influencing tameness and aggression than by an approach that assumes fixation of causative alleles, suggesting that many causative loci were not driven to fixation by the selection. We used RNA sequencing in 150 F2 animals to identify hundreds of loci that influence brain gene expression. Several of these loci colocalize with tameness loci and may reflect the same genetic variants. Through analyses of correlations between allele effects on behavior and gene expression, differential expression between the tame and aggressive rat selection lines, and correlations between gene expression and tameness in F2 animals, we identify the genes Gltscr2, Lgi4, Zfp40, and Slc17a7 as candidate contributors to the strikingly different behavior of the tame and aggressive animals.
AB - Interindividual differences in many behaviors are partly due to genetic differences, but the identification of the genes and variants that influence behavior remains challenging. Here, we studied an F2 intercross of two outbred lines of rats selected for tame and aggressive behavior toward humans for .64 generations. By using a mapping approach that is able to identify genetic loci segregating within the lines, we identified four times more loci influencing tameness and aggression than by an approach that assumes fixation of causative alleles, suggesting that many causative loci were not driven to fixation by the selection. We used RNA sequencing in 150 F2 animals to identify hundreds of loci that influence brain gene expression. Several of these loci colocalize with tameness loci and may reflect the same genetic variants. Through analyses of correlations between allele effects on behavior and gene expression, differential expression between the tame and aggressive rat selection lines, and correlations between gene expression and tameness in F2 animals, we identify the genes Gltscr2, Lgi4, Zfp40, and Slc17a7 as candidate contributors to the strikingly different behavior of the tame and aggressive animals.
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U2 - 10.1534/genetics.114.168948
DO - 10.1534/genetics.114.168948
M3 - Article
C2 - 25189874
AN - SCOPUS:84908627915
SN - 0016-6731
VL - 198
SP - 1277
EP - 1290
JO - Genetics
JF - Genetics
IS - 3
ER -