Genetic Mapping of MAPK-Mediated Complex Traits Across S. cerevisiae

Sebastian Treusch, Frank W. Albert, Joshua S. Bloom, Iulia E. Kotenko, Leonid Kruglyak

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Signaling pathways enable cells to sense and respond to their environment. Many cellular signaling strategies are conserved from fungi to humans, yet their activity and phenotypic consequences can vary extensively among individuals within a species. A systematic assessment of the impact of naturally occurring genetic variation on signaling pathways remains to be conducted. In S. cerevisiae, both response and resistance to stressors that activate signaling pathways differ between diverse isolates. Here, we present a quantitative trait locus (QTL) mapping approach that enables us to identify genetic variants underlying such phenotypic differences across the genetic and phenotypic diversity of S. cerevisiae. Using a Round-robin cross between twelve diverse strains, we identified QTL that influence phenotypes critically dependent on MAPK signaling cascades. Genetic variants under these QTL fall within MAPK signaling networks themselves as well as other interconnected signaling pathways. Finally, we demonstrate how the mapping results from multiple strain background can be leveraged to narrow the search space of causal genetic variants.

Original languageEnglish (US)
Article numbere1004913
JournalPLoS genetics
Issue number1
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 Treusch et al.

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