Genetic variation in ESR2 and estrogen receptor-beta expression in lung tumors

Ji Young Song; Jill M. Siegfried; Brenda Diergaarde; Stephanie R. Land; Robert Bowser; Laura P. Stabile; Sanja Dacic; Rajiv Dhir; Tomoko Nukui; Marjorie Romkes; Joel L. Weissfeld

doi:10.1016/j.canep.2013.03.020

Genetic variation in ESR2 and estrogen receptor-beta expression in lung tumors

Ji Young Song, Jill M. Siegfried, Brenda Diergaarde, Stephanie R. Land, Robert Bowser, Laura P. Stabile, Sanja Dacic, Rajiv Dhir, Tomoko Nukui, Marjorie Romkes, Joel L. Weissfeld

Pharmacology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Objective: To investigate the association between inherited variation in the estrogen receptor beta (ERβ) gene (ESR2) and ERβ lung tumor expression, a phenotype that possibly affects survival differently in men and women. Methods: We genotyped 135 lung cancer patients for 22 ESR2 single nucleotide polymorphisms (SNPs) and measured nuclear and cytoplasmic ERβ expression by immunohistochemistry (IHC) in their primary lung tumor. Distributing Allred ERβ IHC scores according to ESR2 genotype classified under a dominant genetic model, we used rank sum tests to identify ESR2 SNPs significantly associated (p < 0.05) with ERβ expression. Results: 35%, 35%, and 29% of lung tumors showed no/low (Allred < 6), intermediate (Allred 6-7), and maximal (Allred 8) cytoplasmic ERβ expression, whereas 13%, 27%, and 60% showed no/low, intermediate, and maximal nuclear ERβ expression. For SNPs rs8021944, rs1256061 and rs10146204, ERβ expression was higher according to the rank sum test in lung tumors from patients with at least one minor allele. For each of these three SNPs, the odds of maximal (Allred 8) relative to no/low (Allred < 6) ERβ expression was 3-fold higher in tumors from patients with at least one minor allele than in tumors from patients homozygous for the common allele. Conclusion: Inherited variability in ESR2 may determine ERβ lung tumor expression.

Original language	English (US)
Pages (from-to)	518-522
Number of pages	5
Journal	Cancer Epidemiology
Volume	37
Issue number	4
DOIs	https://doi.org/10.1016/j.canep.2013.03.020
State	Published - Aug 2013

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health grants P50 CA090440 , R25 CA057703 , and P30 CA047904 . Study sponsors did not play any role in the study design, in the collection, analysis or interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.

Keywords

Estrogen receptor
Genetic polymorphism
Lung cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Genetic variation in ESR2 and estrogen receptor-beta expression in lung tumors",

abstract = "Objective: To investigate the association between inherited variation in the estrogen receptor beta (ERβ) gene (ESR2) and ERβ lung tumor expression, a phenotype that possibly affects survival differently in men and women. Methods: We genotyped 135 lung cancer patients for 22 ESR2 single nucleotide polymorphisms (SNPs) and measured nuclear and cytoplasmic ERβ expression by immunohistochemistry (IHC) in their primary lung tumor. Distributing Allred ERβ IHC scores according to ESR2 genotype classified under a dominant genetic model, we used rank sum tests to identify ESR2 SNPs significantly associated (p < 0.05) with ERβ expression. Results: 35%, 35%, and 29% of lung tumors showed no/low (Allred < 6), intermediate (Allred 6-7), and maximal (Allred 8) cytoplasmic ERβ expression, whereas 13%, 27%, and 60% showed no/low, intermediate, and maximal nuclear ERβ expression. For SNPs rs8021944, rs1256061 and rs10146204, ERβ expression was higher according to the rank sum test in lung tumors from patients with at least one minor allele. For each of these three SNPs, the odds of maximal (Allred 8) relative to no/low (Allred < 6) ERβ expression was 3-fold higher in tumors from patients with at least one minor allele than in tumors from patients homozygous for the common allele. Conclusion: Inherited variability in ESR2 may determine ERβ lung tumor expression.",

keywords = "Estrogen receptor, Genetic polymorphism, Lung cancer",

author = "Song, {Ji Young} and Siegfried, {Jill M.} and Brenda Diergaarde and Land, {Stephanie R.} and Robert Bowser and Stabile, {Laura P.} and Sanja Dacic and Rajiv Dhir and Tomoko Nukui and Marjorie Romkes and Weissfeld, {Joel L.}",

note = "Funding Information: This work was supported by National Institutes of Health grants P50 CA090440 , R25 CA057703 , and P30 CA047904 . Study sponsors did not play any role in the study design, in the collection, analysis or interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication. ",

year = "2013",

month = aug,

doi = "10.1016/j.canep.2013.03.020",

language = "English (US)",

volume = "37",

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T1 - Genetic variation in ESR2 and estrogen receptor-beta expression in lung tumors

AU - Song, Ji Young

AU - Siegfried, Jill M.

AU - Diergaarde, Brenda

AU - Land, Stephanie R.

AU - Bowser, Robert

AU - Stabile, Laura P.

AU - Dacic, Sanja

AU - Dhir, Rajiv

AU - Nukui, Tomoko

AU - Romkes, Marjorie

AU - Weissfeld, Joel L.

N1 - Funding Information: This work was supported by National Institutes of Health grants P50 CA090440 , R25 CA057703 , and P30 CA047904 . Study sponsors did not play any role in the study design, in the collection, analysis or interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.

PY - 2013/8

Y1 - 2013/8

N2 - Objective: To investigate the association between inherited variation in the estrogen receptor beta (ERβ) gene (ESR2) and ERβ lung tumor expression, a phenotype that possibly affects survival differently in men and women. Methods: We genotyped 135 lung cancer patients for 22 ESR2 single nucleotide polymorphisms (SNPs) and measured nuclear and cytoplasmic ERβ expression by immunohistochemistry (IHC) in their primary lung tumor. Distributing Allred ERβ IHC scores according to ESR2 genotype classified under a dominant genetic model, we used rank sum tests to identify ESR2 SNPs significantly associated (p < 0.05) with ERβ expression. Results: 35%, 35%, and 29% of lung tumors showed no/low (Allred < 6), intermediate (Allred 6-7), and maximal (Allred 8) cytoplasmic ERβ expression, whereas 13%, 27%, and 60% showed no/low, intermediate, and maximal nuclear ERβ expression. For SNPs rs8021944, rs1256061 and rs10146204, ERβ expression was higher according to the rank sum test in lung tumors from patients with at least one minor allele. For each of these three SNPs, the odds of maximal (Allred 8) relative to no/low (Allred < 6) ERβ expression was 3-fold higher in tumors from patients with at least one minor allele than in tumors from patients homozygous for the common allele. Conclusion: Inherited variability in ESR2 may determine ERβ lung tumor expression.

AB - Objective: To investigate the association between inherited variation in the estrogen receptor beta (ERβ) gene (ESR2) and ERβ lung tumor expression, a phenotype that possibly affects survival differently in men and women. Methods: We genotyped 135 lung cancer patients for 22 ESR2 single nucleotide polymorphisms (SNPs) and measured nuclear and cytoplasmic ERβ expression by immunohistochemistry (IHC) in their primary lung tumor. Distributing Allred ERβ IHC scores according to ESR2 genotype classified under a dominant genetic model, we used rank sum tests to identify ESR2 SNPs significantly associated (p < 0.05) with ERβ expression. Results: 35%, 35%, and 29% of lung tumors showed no/low (Allred < 6), intermediate (Allred 6-7), and maximal (Allred 8) cytoplasmic ERβ expression, whereas 13%, 27%, and 60% showed no/low, intermediate, and maximal nuclear ERβ expression. For SNPs rs8021944, rs1256061 and rs10146204, ERβ expression was higher according to the rank sum test in lung tumors from patients with at least one minor allele. For each of these three SNPs, the odds of maximal (Allred 8) relative to no/low (Allred < 6) ERβ expression was 3-fold higher in tumors from patients with at least one minor allele than in tumors from patients homozygous for the common allele. Conclusion: Inherited variability in ESR2 may determine ERβ lung tumor expression.

KW - Estrogen receptor

KW - Genetic polymorphism

KW - Lung cancer

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JF - Cancer Epidemiology

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Genetic variation in ESR2 and estrogen receptor-beta expression in lung tumors

Abstract

Bibliographical note

Keywords

UN SDGs

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