Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm

J. Massman; B. Cooper; R. Horsley; S. Neate; R. Dill-Macky; S. Chao; Y. Dong; P. Schwarz; G. J. Muehlbauer; K. P. Smith

doi:10.1007/s11032-010-9442-0

Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm

J. Massman, B. Cooper, R. Horsley, S. Neate, R. Dill-Macky, S. Chao, Y. Dong, P. Schwarz, G. J. Muehlbauer, K. P. Smith

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130 Scopus citations

Abstract

Utilization of quantitative trait loci (QTL) identified in bi-parental mapping populations has had limited success for improving complex quantitative traits with low to moderate heritability. Association mapping in contemporary breeding germplasm may lead to more effective marker strategies for crop improvement. To test this approach, we conducted association mapping of two complex traits with moderate heritability; Fusarium head blight (FHB) severity and the grain concentration of mycotoxin associated with disease, deoxynivalenol (DON). To map FHB resistance in barley, 768 breeding lines were evaluated in 2006 and 2007 in four locations. All lines were genotyped with 1,536 SNP markers and QTL were mapped using a mixed model that accounts for relatedness among lines. Average linkage disequilibrium within the breeding germplasm extended beyond 4 cM. Four QTL were identified for FHB severity and eight QTL were identified for the DON concentration in two independent sets of breeding lines. The QTL effects were small, explaining 1-3% of the phenotypic variation, as might be expected for complex polygenic traits. We show that using breeding germplasm to map QTL can complement bi-parental mapping studies by providing independent validation, mapping QTL with more precision, resolving questions of linkage and pleiotropy, and identifying genetic markers that can be applied immediately in crop improvement.

Original language	English (US)
Pages (from-to)	439-454
Number of pages	16
Journal	Molecular Breeding
Volume	27
Issue number	4
DOIs	https://doi.org/10.1007/s11032-010-9442-0
State	Published - Apr 2011

Bibliographical note

Funding Information:
Acknowledgments This research was supported by The U.S. Wheat and Barley Scab Initiative Grant No. 59-0790-4-120, the Minnesota Agricultural Experiment Station, and USDA-CSREES-NRI Grant No. 2006-55606-16722 ‘‘Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement.’’.

Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.

Keywords

Association mapping
Barley
Deoxynivalenol
Disease resistance
Fusarium head blight
Linkage disequilibrium
Quantitative trait loci

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title = "Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm",

abstract = "Utilization of quantitative trait loci (QTL) identified in bi-parental mapping populations has had limited success for improving complex quantitative traits with low to moderate heritability. Association mapping in contemporary breeding germplasm may lead to more effective marker strategies for crop improvement. To test this approach, we conducted association mapping of two complex traits with moderate heritability; Fusarium head blight (FHB) severity and the grain concentration of mycotoxin associated with disease, deoxynivalenol (DON). To map FHB resistance in barley, 768 breeding lines were evaluated in 2006 and 2007 in four locations. All lines were genotyped with 1,536 SNP markers and QTL were mapped using a mixed model that accounts for relatedness among lines. Average linkage disequilibrium within the breeding germplasm extended beyond 4 cM. Four QTL were identified for FHB severity and eight QTL were identified for the DON concentration in two independent sets of breeding lines. The QTL effects were small, explaining 1-3% of the phenotypic variation, as might be expected for complex polygenic traits. We show that using breeding germplasm to map QTL can complement bi-parental mapping studies by providing independent validation, mapping QTL with more precision, resolving questions of linkage and pleiotropy, and identifying genetic markers that can be applied immediately in crop improvement.",

keywords = "Association mapping, Barley, Deoxynivalenol, Disease resistance, Fusarium head blight, Linkage disequilibrium, Quantitative trait loci",

author = "J. Massman and B. Cooper and R. Horsley and S. Neate and R. Dill-Macky and S. Chao and Y. Dong and P. Schwarz and Muehlbauer, {G. J.} and Smith, {K. P.}",

note = "Funding Information: Acknowledgments This research was supported by The U.S. Wheat and Barley Scab Initiative Grant No. 59-0790-4-120, the Minnesota Agricultural Experiment Station, and USDA-CSREES-NRI Grant No. 2006-55606-16722 {\textquoteleft}{\textquoteleft}Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement.{\textquoteright}{\textquoteright}. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

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AU - Dill-Macky, R.

AU - Chao, S.

AU - Dong, Y.

AU - Schwarz, P.

AU - Muehlbauer, G. J.

AU - Smith, K. P.

N1 - Funding Information: Acknowledgments This research was supported by The U.S. Wheat and Barley Scab Initiative Grant No. 59-0790-4-120, the Minnesota Agricultural Experiment Station, and USDA-CSREES-NRI Grant No. 2006-55606-16722 ‘‘Barley Coordinated Agricultural Project: Leveraging Genomics, Genetics, and Breeding for Gene Discovery and Barley Improvement.’’. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

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N2 - Utilization of quantitative trait loci (QTL) identified in bi-parental mapping populations has had limited success for improving complex quantitative traits with low to moderate heritability. Association mapping in contemporary breeding germplasm may lead to more effective marker strategies for crop improvement. To test this approach, we conducted association mapping of two complex traits with moderate heritability; Fusarium head blight (FHB) severity and the grain concentration of mycotoxin associated with disease, deoxynivalenol (DON). To map FHB resistance in barley, 768 breeding lines were evaluated in 2006 and 2007 in four locations. All lines were genotyped with 1,536 SNP markers and QTL were mapped using a mixed model that accounts for relatedness among lines. Average linkage disequilibrium within the breeding germplasm extended beyond 4 cM. Four QTL were identified for FHB severity and eight QTL were identified for the DON concentration in two independent sets of breeding lines. The QTL effects were small, explaining 1-3% of the phenotypic variation, as might be expected for complex polygenic traits. We show that using breeding germplasm to map QTL can complement bi-parental mapping studies by providing independent validation, mapping QTL with more precision, resolving questions of linkage and pleiotropy, and identifying genetic markers that can be applied immediately in crop improvement.

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Genome-wide association mapping of Fusarium head blight resistance in contemporary barley breeding germplasm

Abstract

Bibliographical note

Keywords

UN SDGs

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