Genome-wide detection of genetic loci associated with soybean aphid resistance in soybean germplasm PI 603712

Siddhi J. Bhusal; Guo Liang Jiang; Qijian Song; Perry B. Cregan; David Wright; Jose L. Gonzalez-Hernandez

doi:10.1007/s10681-017-1933-1

Genome-wide detection of genetic loci associated with soybean aphid resistance in soybean germplasm PI 603712

Siddhi J. Bhusal, Guo Liang Jiang, Qijian Song, Perry B. Cregan, David Wright, Jose L. Gonzalez-Hernandez

Agronomy and Plant Genetics

Research output: Contribution to journal › Article › peer-review

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Abstract

Soybean aphid (Aphis glycines Matsumura) has become one of the major pests of soybean [Glycine max (L.) Merr.] in North America since 2000. At least four biotypes of soybean aphid have been confirmed in the United States. Genetic characterization of new sources of soybean aphid resistance will facilitate the expansion of soybean gene pool for soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To characterize the genetic basis of soybean aphid resistance in PI 603712, a newly identified resistant germplasm line, 142 F₂ plants derived from the cross ‘Roberts’ × PI 603712 and their parents were evaluated for soybean aphid resistance in the greenhouse, and were genotyped with BARCSoySNP6K Illumina Infinium II BeadChip. A genome-wide molecular linkage map was constructed with 1495 polymorphic SNP markers. QTL analysis revealed that PI 603712 possessed two major loci associated with soybean aphid resistance, located on chromosome 7 and 16, respectively. The locus on chromosome 7 was dominantly expressed and positioned about one Mega-base-pair distant from the previously identified resistance locus Rag1. The locus on chromosome 16 was positioned near the previously identified resistance locus Rag3 and expressed partially dominance or additive effect. Interestingly, two minor loci were also detected on chromosomes 13 and 17 but the alleles from PI 603712 decreased the resistance. In developing soybean aphid resistant cultivars through marker-assisted selection, an appropriate combination of resistance loci should be selected when PI 603712 is used as a donor parent of resistance.

Original language	English (US)
Article number	144
Journal	Euphytica
Volume	213
Issue number	7
DOIs	https://doi.org/10.1007/s10681-017-1933-1
State	Published - Jul 1 2017

Bibliographical note

Funding Information:
This study was financially supported in part by the grants of the United Soybean Board and the South Dakota Soybean Research and Promotion Council awarded to Guo-Liang Jiang. USDA-NIFA/CSREES Hatch project through South Dakota Agricultural Experiment Station and Virginia State University Agricultural Research Station also provided support in completion of the research and manuscript. The authors are thankful to Dr. Kelley Tilmon and her lab for assistance in soybean aphid colony rearing and resistance screening; Dr. Richard Joost, Director of Supply Programs of the United Soybean Board, for review and comments; and Dr. Xianzhi Wang, Dr. Jiaoping Zhang, Dr. Mukhtar Agoub, and Mr. Nicholas Hall for assistance in lab and greenhouse experiments.

Publisher Copyright:
© 2017, Springer Science+Business Media B.V.

Keywords

Linkage map
QTL analysis
Resistance locus
Soybean aphid

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Genome-wide detection of genetic loci associated with soybean aphid resistance in soybean germplasm PI 603712",

abstract = "Soybean aphid (Aphis glycines Matsumura) has become one of the major pests of soybean [Glycine max (L.) Merr.] in North America since 2000. At least four biotypes of soybean aphid have been confirmed in the United States. Genetic characterization of new sources of soybean aphid resistance will facilitate the expansion of soybean gene pool for soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To characterize the genetic basis of soybean aphid resistance in PI 603712, a newly identified resistant germplasm line, 142 F2 plants derived from the cross {\textquoteleft}Roberts{\textquoteright} × PI 603712 and their parents were evaluated for soybean aphid resistance in the greenhouse, and were genotyped with BARCSoySNP6K Illumina Infinium II BeadChip. A genome-wide molecular linkage map was constructed with 1495 polymorphic SNP markers. QTL analysis revealed that PI 603712 possessed two major loci associated with soybean aphid resistance, located on chromosome 7 and 16, respectively. The locus on chromosome 7 was dominantly expressed and positioned about one Mega-base-pair distant from the previously identified resistance locus Rag1. The locus on chromosome 16 was positioned near the previously identified resistance locus Rag3 and expressed partially dominance or additive effect. Interestingly, two minor loci were also detected on chromosomes 13 and 17 but the alleles from PI 603712 decreased the resistance. In developing soybean aphid resistant cultivars through marker-assisted selection, an appropriate combination of resistance loci should be selected when PI 603712 is used as a donor parent of resistance.",

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note = "Funding Information: This study was financially supported in part by the grants of the United Soybean Board and the South Dakota Soybean Research and Promotion Council awarded to Guo-Liang Jiang. USDA-NIFA/CSREES Hatch project through South Dakota Agricultural Experiment Station and Virginia State University Agricultural Research Station also provided support in completion of the research and manuscript. The authors are thankful to Dr. Kelley Tilmon and her lab for assistance in soybean aphid colony rearing and resistance screening; Dr. Richard Joost, Director of Supply Programs of the United Soybean Board, for review and comments; and Dr. Xianzhi Wang, Dr. Jiaoping Zhang, Dr. Mukhtar Agoub, and Mr. Nicholas Hall for assistance in lab and greenhouse experiments. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media B.V.",

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AU - Cregan, Perry B.

AU - Wright, David

AU - Gonzalez-Hernandez, Jose L.

N1 - Funding Information: This study was financially supported in part by the grants of the United Soybean Board and the South Dakota Soybean Research and Promotion Council awarded to Guo-Liang Jiang. USDA-NIFA/CSREES Hatch project through South Dakota Agricultural Experiment Station and Virginia State University Agricultural Research Station also provided support in completion of the research and manuscript. The authors are thankful to Dr. Kelley Tilmon and her lab for assistance in soybean aphid colony rearing and resistance screening; Dr. Richard Joost, Director of Supply Programs of the United Soybean Board, for review and comments; and Dr. Xianzhi Wang, Dr. Jiaoping Zhang, Dr. Mukhtar Agoub, and Mr. Nicholas Hall for assistance in lab and greenhouse experiments. Publisher Copyright: © 2017, Springer Science+Business Media B.V.

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N2 - Soybean aphid (Aphis glycines Matsumura) has become one of the major pests of soybean [Glycine max (L.) Merr.] in North America since 2000. At least four biotypes of soybean aphid have been confirmed in the United States. Genetic characterization of new sources of soybean aphid resistance will facilitate the expansion of soybean gene pool for soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To characterize the genetic basis of soybean aphid resistance in PI 603712, a newly identified resistant germplasm line, 142 F2 plants derived from the cross ‘Roberts’ × PI 603712 and their parents were evaluated for soybean aphid resistance in the greenhouse, and were genotyped with BARCSoySNP6K Illumina Infinium II BeadChip. A genome-wide molecular linkage map was constructed with 1495 polymorphic SNP markers. QTL analysis revealed that PI 603712 possessed two major loci associated with soybean aphid resistance, located on chromosome 7 and 16, respectively. The locus on chromosome 7 was dominantly expressed and positioned about one Mega-base-pair distant from the previously identified resistance locus Rag1. The locus on chromosome 16 was positioned near the previously identified resistance locus Rag3 and expressed partially dominance or additive effect. Interestingly, two minor loci were also detected on chromosomes 13 and 17 but the alleles from PI 603712 decreased the resistance. In developing soybean aphid resistant cultivars through marker-assisted selection, an appropriate combination of resistance loci should be selected when PI 603712 is used as a donor parent of resistance.

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Genome-wide detection of genetic loci associated with soybean aphid resistance in soybean germplasm PI 603712

Abstract

Bibliographical note

Keywords

UN SDGs

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