Genomic analysis of genetic markers associated with inherited cardiomyopathy (round heart disease) in the turkey (Meleagris gallopavo)

Kent Reed, K. M. Mendoza, G. R. Hu, L. R. Sullivan, M. W. Grace, L. D. Chaves, D. L. Kooyman

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In turkeys, spontaneous cardiomyopathy or round heart (RH) disease is characterised by dilated ventricles and cardiac muscle hypertrophy. Although the aetiology of RH is still unknown, the disease can have a significant economic impact on turkey producers. In an initial attempt to identify genomic regions associated with RH, we utilised the chicken genome sequence to target short DNA sequences (sequence-characterised amplified regions, SCARs) identified in previous studies that had significant differences in frequency distribution between RH+ and RH- turkeys. SCARs were comparatively aligned with the chicken whole-genome sequence to identify flanking regions for primer design. Primers from 32 alignments were tested and target sequences were successfully amplified for 30 loci (94%). Comparative re-sequencing identified putative SNPs in 20 of the 30 loci (67%). Genetically informative SNPs at 16 loci were genotyped in the UMN/NTBF turkey mapping population. As a result of this study, 34 markers were placed on the turkey/chicken comparative map and 15 markers were added to the turkey genetic linkage map. The position of these markers relative to cardiac-related genes is presented. In addition, analysis of genotypes at 109 microsatellite loci presumed to flank the SCAR sequences in the turkey genome identified four significant associations with RH.

Original languageEnglish (US)
Pages (from-to)211-217
Number of pages7
JournalAnimal Genetics
Volume38
Issue number3
DOIs
StatePublished - Jun 1 2007

Keywords

  • Cardiomyopathy
  • Genetic mapping
  • Meleagris gallopavo
  • Round heart
  • SNP

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