We have isolated and characterized overlapping genomic clones containing the complete transcribed region of a newly isolated mouse cDNA encoding an orphan receptor expressed specifically in midgestation embryos and adult testis. This gene spans a distance of more than 50 kb and is organized into 13 exons. The transcription initiation site is located at the 158th nucleotide upstream from the translation initiation codon. All the exon/intron junction sequences follow the GT/AG rule. Based upon Northern blot analysis and the size of the transcribed region of the gene, its transcript was determined to be approximately 2.5 kb. Within approximately 500 bp upstream from the transcription initiation site, several immune response regulatory elements were identified but no TATA box was located. This gene was mapped to the distal region of mouse chromosome 10 and its locus has been designatedTr2-11.Immunohistochemical studies show that the Tr2-11 protein is present mainly in advanced germ cell populations of mature testes and thatTr2-11gene expression is dramatically decreased in vitamin A-depleted animals.
Bibliographical noteFunding Information:
This work was supported by Grants DK46866±01 and SMF-975± 95 and a Grant-in-Aid of Research, Artistry, and Scholarship from the Graduate School of the University of Minnesota to L.-N.W. and in part by the National Cancer Institute, DHHS, under Contract N01-CO-46000 with ABL. We thank Dr. Liming Chang for help in antibody puri®cation and Debbie Barnhart for excellent technical assistance. We thank Dr. K. P. Roberts for a critical reading of the manuscript. We also thank Drs. D. Hamilton, K. P. Roberts, and J. E. Siiteri for their help in testis immunohistochemistry.
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