Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU

Errol Reiss; Brent A. Lasker; Timothy J. Lott; Catherine M Bendel; David A. Kaufman; Kevin C. Hazen; Kelly C. Wade; Karin L. McGowan; Shawn R. Lockhart

doi:10.1016/j.meegid.2012.06.012

Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU

Errol Reiss, Brent A. Lasker, Timothy J. Lott, Catherine M Bendel, David A. Kaufman, Kevin C. Hazen, Kelly C. Wade, Karin L. McGowan, Shawn R. Lockhart

Pediatrics - Neonatology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Candida parapsilosis (CP) (n= 40) isolated from an unselected patient population in the neonatal intensive care units (NICUs) of three US hospitals were collected over periods of 3.5-9. years. Two previously published microsatellite markers and three additional trinucleotide markers were used to produce multiplex genotypes, which revealed broad strain diversity among the NICU isolates with a combined index of discrimination (D) = 0.997. A cluster of eight related CP strains from four infants in a single NICU was observed. An extended collection of 24 CP isolates from the general population of that hospital showed that the cluster of NICU isolates was related to three isolates from general hospital patients. This microsatellite marker set is suitable to investigate clusters of colonizing and infecting strains of CP.

Original language	English (US)
Pages (from-to)	1654-1660
Number of pages	7
Journal	Infection, Genetics and Evolution
Volume	12
Issue number	8
DOIs	https://doi.org/10.1016/j.meegid.2012.06.012
State	Published - Dec 2012

Keywords

Candida parapsilosis
Microsatellite
Molecular epidemiology
Neonatal intensive care unit

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Reiss, E., Lasker, B. A., Lott, T. J., Bendel, C. M., Kaufman, D. A., Hazen, K. C., Wade, K. C., McGowan, K. L., & Lockhart, S. R. (2012). Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU. Infection, Genetics and Evolution, 12(8), 1654-1660. https://doi.org/10.1016/j.meegid.2012.06.012

Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU. / Reiss, Errol; Lasker, Brent A.; Lott, Timothy J. et al.
In: Infection, Genetics and Evolution, Vol. 12, No. 8, 12.2012, p. 1654-1660.

Research output: Contribution to journal › Article › peer-review

Reiss, E, Lasker, BA, Lott, TJ, Bendel, CM, Kaufman, DA, Hazen, KC, Wade, KC, McGowan, KL & Lockhart, SR 2012, 'Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU', Infection, Genetics and Evolution, vol. 12, no. 8, pp. 1654-1660. https://doi.org/10.1016/j.meegid.2012.06.012

Reiss E, Lasker BA, Lott TJ, Bendel CM, Kaufman DA, Hazen KC et al. Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU. Infection, Genetics and Evolution. 2012 Dec;12(8):1654-1660. doi: 10.1016/j.meegid.2012.06.012

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abstract = "Candida parapsilosis (CP) (n= 40) isolated from an unselected patient population in the neonatal intensive care units (NICUs) of three US hospitals were collected over periods of 3.5-9. years. Two previously published microsatellite markers and three additional trinucleotide markers were used to produce multiplex genotypes, which revealed broad strain diversity among the NICU isolates with a combined index of discrimination (D) = 0.997. A cluster of eight related CP strains from four infants in a single NICU was observed. An extended collection of 24 CP isolates from the general population of that hospital showed that the cluster of NICU isolates was related to three isolates from general hospital patients. This microsatellite marker set is suitable to investigate clusters of colonizing and infecting strains of CP.",

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Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU

Abstract

Keywords

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