Gentle dielectrophoretic focusing of yeast cells in curved microchannels

Christopher Church, Gaoyan Wang, Junjie Zhu, Tzuen Rong Jeremy Tzeng, Xiangchun Xuan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Focusing cells into a tight stream is usually a necessary step prior to counting, detecting and sorting them in, for example, microfluidic flow cytometers. We present herein a simple and gentle cell focusing technique in physiological solutions through a serpentine microchannel using DC-biased AC electric fields. This electrokinetic focusing eliminates sheath flows and in-channel microelectrodes. It results from the cross-stream dielectrophoretic motion of cells induced by the intrinsic channel curvatures. The effects of electric field magnitude, AC to DC electric field ratio, AC field frequency, and cell concentration on the focusing performance of yeast cells will be studied.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Pages283-287
Number of pages5
DOIs
StatePublished - Jul 12 2010
EventASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009 - Shanghai, China
Duration: Dec 18 2009Dec 21 2009

Publication series

NameProceedings of the ASME Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
Volume1

Other

OtherASME 2009 Micro/Nanoscale Heat and Mass Transfer International Conference 2009, MNHMT2009
CountryChina
CityShanghai
Period12/18/0912/21/09

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