Glioma cell density in a rat gene therapy model gauged by water relaxation rate along a fictitious magnetic field

Timo Liimatainen, Alejandra Sierra, Timothy Hanson, Dennis J. Sorce, Seppo Ylä-Herttuala, Michael Garwood, Shalom Michaeli, Olli Gröhn

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Longitudinal and transverse rotating-frame relaxation time constants, T and T, have previously been successfully applied to detect gene therapy responses and acute stroke in animal models. Those experiments were performed with continuous-wave irradiation or with frequency-modulated pulses operating in an adiabatic regime. The technique called Relaxation Along a Fictitious Field (RAFF) is a recent extension of frequency-modulated rotating-frame relaxation methods. In RAFF, spin locking takes place along a fictitious magnetic field, and the decay rate is a function of both T and T processes. In this work, the time constant characterizing water relaxation with RAFF (T RAFF) was evaluated for its utility as a marker of response to gene therapy in a rat glioma model. To investigate the sensitivity to early treatment response, we measured several rotating-frame and free-precession relaxation time constants and the water apparent diffusion coefficients, and these were compared with histological cell counts in 8 days of treated and control groups of animals. TRAFF was the only parameter exhibiting significant association with cell density in three different tumor regions (border, intermediate, and core tissues). These results indicate that TRAFF may provide a marker to identify tumors responding to treatment.

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
JournalMagnetic resonance in medicine
Volume67
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • contrast imaging
  • fictitious field
  • gene therapy
  • glioma
  • rotating frame relaxation

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