Glucose-based poly(ester amines): Synthesis, degradation, and biological delivery

Chen Chang Lee; Yemin Liu; Theresa M. Reineke

doi:10.1021/mz300505t

Glucose-based poly(ester amines): Synthesis, degradation, and biological delivery

Chen Chang Lee, Yemin Liu, Theresa M. Reineke

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Herein, the synthesis and characterization of two glucose-based degradable poly(ester amines), GluN3 and GluN4, are described. Data from gel shift assays, PicoGreen dye exclusion, and dynamic light scattering studies reveals that these polycations can form polyplexes with plasmid DNA at a relatively low N/P ratio and that the polymers degrade rapidly at physiological pH conditions. Assessment of in vitro data via flow cytometry and live cell fluorescence microscopy indicated that GluN3 and GluN4 polyplexes can be internalized by HeLa cells in a highly effective manner with low cytotoxicity profiles.

Original language	English (US)
Pages (from-to)	1388-1392
Number of pages	5
Journal	ACS Macro Letters
Volume	1
Issue number	12
DOIs	https://doi.org/10.1021/mz300505t
State	Published - Dec 18 2012

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title = "Glucose-based poly(ester amines): Synthesis, degradation, and biological delivery",

abstract = "Herein, the synthesis and characterization of two glucose-based degradable poly(ester amines), GluN3 and GluN4, are described. Data from gel shift assays, PicoGreen dye exclusion, and dynamic light scattering studies reveals that these polycations can form polyplexes with plasmid DNA at a relatively low N/P ratio and that the polymers degrade rapidly at physiological pH conditions. Assessment of in vitro data via flow cytometry and live cell fluorescence microscopy indicated that GluN3 and GluN4 polyplexes can be internalized by HeLa cells in a highly effective manner with low cytotoxicity profiles.",

author = "Lee, {Chen Chang} and Yemin Liu and Reineke, {Theresa M.}",

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AU - Liu, Yemin

AU - Reineke, Theresa M.

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AB - Herein, the synthesis and characterization of two glucose-based degradable poly(ester amines), GluN3 and GluN4, are described. Data from gel shift assays, PicoGreen dye exclusion, and dynamic light scattering studies reveals that these polycations can form polyplexes with plasmid DNA at a relatively low N/P ratio and that the polymers degrade rapidly at physiological pH conditions. Assessment of in vitro data via flow cytometry and live cell fluorescence microscopy indicated that GluN3 and GluN4 polyplexes can be internalized by HeLa cells in a highly effective manner with low cytotoxicity profiles.

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Glucose-based poly(ester amines): Synthesis, degradation, and biological delivery

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