GPCR-dependent biasing of GIRK channel signaling dynamics by RGS6 in mouse sinoatrial nodal cells

Allison Anderson, Ikuo Masuho, Ezequiel Marron Fernandez De Velasco, Atsushi Nakano, Lutz Birnbaumer, Lutz Birnbaumer, Kirill A. Martemyanov, Kevin Wickman

Research output: Contribution to journalArticlepeer-review

Abstract

How G protein-coupled receptors (GPCRs) evoke specific biological outcomes while utilizing a limited array of G proteins and effectors is poorly understood, particularly in native cell systems. Here, we examined signaling evoked by muscarinic (M2R) and adenosine (A1R) receptor activation in the mouse sinoatrial node (SAN), the cardiac pacemaker. M2R and A1R activate a shared pool of cardiac G protein-gated inwardly rectifying K+ (GIRK) channels in SAN cells from adult mice, but A1R-GIRK responses are smaller and slower than M2R-GIRK responses. Recordings from mice lacking Regulator of G protein Signaling 6 (RGS6) revealed that RGS6 exerts a GPCRdependent influence on GIRK-dependent signaling in SAN cells, suppressing M2R-GIRK coupling efficiency and kinetics and A1R-GIRK signaling amplitude. Fast kinetic bioluminescence resonance energy transfer assays in transfected HEK cells showed that RGS6 prefers Gαoover Gαi as a substrate for its catalytic activity and that M2R signals preferentially via Gαo, while A1R does not discriminate between inhibitory G protein isoforms. The impact of atrial/SAN-selective ablation of Gαoor Gai2 was consistent with these findings. Gai2ablation hadminimal impact onM2R-GIRK and A1R-GIRK signaling in SAN cells. In contrast, Gαoablation decreased the amplitude and slowed the kinetics of M2R-GIRK responses, while enhancing the sensitivity and prolonging the deactivation rate of A1R-GIRK signaling. Collectively, our data show that differences in GPCR-G protein coupling preferences, and the Gαosubstrate preference of RGS6, shape A1R- and M2R-GIRK signaling dynamics in mouse SAN cells.

Original languageEnglish (US)
Pages (from-to)14522-14531
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number25
DOIs
StatePublished - Jun 23 2020

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS. We thank Dr. C. David Weaver and Dr. Corey Hopkins for providing ML297, Nickolas Skamangas for technical support, and Hannah Oberle and Mehrsa Zahiremami for exceptional care of the mouse colony. This work was supported by NIH Grants HL105550 (to K.W. and K.A.M.), R21 HL124503 (to A.N.), and F31 HL139090 (A.A.) and by NIH Intramural Research Program Project Grant Z01-ES-101643 (to L.B.).

Publisher Copyright:
© 2020 National Academy of Sciences. All rights reserved.

Keywords

  • Adenosine
  • G protein
  • Heart rate
  • Kir3
  • Muscarinic

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