GSG1, a yeast gene required for sporulation

Michael D. Kaytor, Dennis M. Livingston

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have identified a gene, GSG1 (general sporulation gene 1), required for sporulation in Saccharomyces cerevisiae. Diploids homozygous for a disruption of GSG1 fail to sporulate. The gene has an open reading frame of 2094 bp, encoding a polypeptide with an expected size of 77 kDa. GSG1 is expressed mitotically in both a and α haploids, and both mitotically and meiotically in diploids. The message level of GSG1 increases approximately two‐fold after 4–6 h of sporulation. gsg1 mutants enter pre‐meiotic DNA synthesis later than wild‐type diploids. Mutant diploids are not rescued by spo13. These results suggest that GSG1 has a role late in meiosis following DNA replication. The sequence reported here has the GenBank Accession Number U26674.

Original languageEnglish (US)
Pages (from-to)1147-1155
Number of pages9
JournalYeast
Volume11
Issue number12
DOIs
StatePublished - Sep 30 1995

Keywords

  • GSG1
  • Saccharomyces cerevisiae
  • meiosis
  • sporulation

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