Guanylate cyclase in the accessory gland of the cricket, Acheta domesticus

Ann Marie Fallon, G. R. Wyatt

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5 Scopus citations

Abstract

Guanylate cyclase (E.C. 4.6.1.2.) was investigated in the accessory reproductive gland of the male house cricket, Acheta domesticus, which is known to accumulate exceptionally high levels of guanosine 3′,5′-cyclic monophosphate (cyclic GMP). Accessory gland guanylate cyclase activity was linear with time for at least one hour, and with enzyme concentration to about 5 mg soluble protein per ml. Activity was dependent on Mn2+ and was maximal at pH 7.3 to 8.0. Sodium fluoride had no effect on activity, but sodium azide was slightly stimulatory. About 80% of the activity was sedimentable at 16,000 g, and both soluble and particulate activities were increased slightly in the presence of Triton X-100. Kinetic analysis indicated half-maximal velocity at 85 μM GTP in the presence of excess Mn2+, and reciprocal plots were concave upward. Changes in activity during maturation of the gland were small, and did not provide evidence for a regulatory role of guanylate cyclase in the accumulation of accessory gland cyclic GMP. The regulation and rôle of cyclic GMP in the accessory gland are discussed.

Original languageEnglish (US)
Pages (from-to)1037-1041
Number of pages5
JournalJournal of Insect Physiology
Volume23
Issue number8
DOIs
StatePublished - 1977

Bibliographical note

Funding Information:
Acknonlrclyetnrrirs-This research was supported by grants from the U.S. National Institutes of Health (HD-0795 1)a nd the National Research Council of Canada.

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