TY - JOUR
T1 - Inibição da guanilato ciclase pelo azul de metileno no choque circulatório causado por pancreatite aguda necrosante
T2 - Uma palavra de cuidado embasada em modelo suíno
AU - Baldo, Caroline Floreoto
AU - Capellini, Verena Kise
AU - Celotto, Andrea Carla
AU - Sônego, Fabiane
AU - Tirapelli, Luis Fernando
AU - Batalhão, Marcelo
AU - Cárnio, Evelin Capellari
AU - dos Santos, José Sebastião
AU - Evora, Paulo Roberto Barbosa
PY - 2013/11
Y1 - 2013/11
N2 - Objective: To study the therapeutic application of guanylate cyclase inhibition by methylene blue in an experimental model of acute pancreatitis in pigs. Methods: acute necrotizing pancreatitis was induced in anesthetized pigs by the retrograde infusion of 1 ml/kg of 5% sodium taurocholate and 8 U/kg enterokinase in the pancreatic duct. Three groups were studied (n = 5): control (C), pancreatitis (AP), and MB bolus followed by pancreatitis (MB+P). The data included serum and abdominal fluid enzymes, hemodynamic variables, arterial hemogasometry, abdominal fluid volume, inflammatory markers, plasma nitrite/nitrate (NOx), plasma myeloperoxidase (MPO) and plasma malondialdehyde (MDA). One and two-way analysis of variance (ANOVA) was performed, followed by the Bonferroni test (p < 0.05). Results: amylase and lipase were three and 10-fold higher in the AP group. Myeloperoxidase activity was 50% higher in the AP group. The hemodynamic data indicated early hypovolemic shock followed by cardiogenic shock. Severe fluid translocation to the peritoneal cavity was observed. Plasma NOx remained unchanged. The MB+P group had a five-fold increase in MDA compared with the C group. Conclusion: preemptive application of MB in pigs with AP demonstrated no significant effects on hemodynamic and inflammatory variables. The use of MB is inadequate in cases of exponential NO release, and extreme caution must be exercised, given the increase in lipid peroxidation based on the malondialdehyde dosage.
AB - Objective: To study the therapeutic application of guanylate cyclase inhibition by methylene blue in an experimental model of acute pancreatitis in pigs. Methods: acute necrotizing pancreatitis was induced in anesthetized pigs by the retrograde infusion of 1 ml/kg of 5% sodium taurocholate and 8 U/kg enterokinase in the pancreatic duct. Three groups were studied (n = 5): control (C), pancreatitis (AP), and MB bolus followed by pancreatitis (MB+P). The data included serum and abdominal fluid enzymes, hemodynamic variables, arterial hemogasometry, abdominal fluid volume, inflammatory markers, plasma nitrite/nitrate (NOx), plasma myeloperoxidase (MPO) and plasma malondialdehyde (MDA). One and two-way analysis of variance (ANOVA) was performed, followed by the Bonferroni test (p < 0.05). Results: amylase and lipase were three and 10-fold higher in the AP group. Myeloperoxidase activity was 50% higher in the AP group. The hemodynamic data indicated early hypovolemic shock followed by cardiogenic shock. Severe fluid translocation to the peritoneal cavity was observed. Plasma NOx remained unchanged. The MB+P group had a five-fold increase in MDA compared with the C group. Conclusion: preemptive application of MB in pigs with AP demonstrated no significant effects on hemodynamic and inflammatory variables. The use of MB is inadequate in cases of exponential NO release, and extreme caution must be exercised, given the increase in lipid peroxidation based on the malondialdehyde dosage.
KW - Free radicals methylene blue
KW - Guanylate cyclase
KW - Nitric oxide
KW - Pancreatitis
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U2 - 10.1590/S0100-69912013000600011
DO - 10.1590/S0100-69912013000600011
M3 - Article
C2 - 24573627
AN - SCOPUS:84897629567
SN - 0100-6991
VL - 40
SP - 480
EP - 489
JO - Revista do Colegio Brasileiro de Cirurgioes
JF - Revista do Colegio Brasileiro de Cirurgioes
IS - 6
ER -